Cy7-labeled W20/XD4-SPIONs or unconjugated-SPIONs were dissolved in PBS containing 15% mannitol for administration. Six nude mice were used in each group. Each mouse was intravenously administrated with 300 μL of CY7-labeled SPIONs via the tail vein (200 μmol Fe/kg body weight). The fluorescence signal was imaged using an IVIS spectrum imaging system (Kodak In-Vivo Imaging System FX Pro, USA) at 1, 2, 4, 8, 12 and 24 h post-administration. The organs of mice including brain, heart, liver, spleen, lungs and kidneys were collected at 0, 2, 4, 12, 24 h post administration, washed with saline, and the specific CY7 tissue fluorescence for each organ was quantified using the IVIS spectrum imaging system.
In vivo imaging system fx pro
Manufactured by Kodak
Sourced in United States
The In-Vivo Imaging System FX Pro is a versatile and high-performance imaging system designed for small animal research. It provides advanced imaging capabilities, allowing researchers to capture and analyze images of living organisms in a non-invasive manner. The system utilizes a range of imaging modalities, including fluorescence, luminescence, and X-ray, enabling comprehensive analysis of biological processes and disease models.
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Lab products found in correlation
Tcs sp2, by Leica (1 mentions)
Smartlab, by Rigaku (1 mentions)
Jem 2000fx, by JEOL (1 mentions)
Fp 8600 fluorospectrophotometer, by Jasco (1 mentions)
Molecular imaging software 5 x, by Kodak (1 mentions)
Mi software, by Carestream (1 mentions)
In vivo imaging system, by Kodak (1 mentions)
Spectramax m5, by Molecular Devices (1 mentions)
Molecular imaging software, by Kodak (1 mentions)
Quantum gx μct, by PerkinElmer (1 mentions)
22 protocols using in vivo imaging system fx pro
1
In Vivo SPION Biodistribution Imaging
2
Aging and Bone Morphology in Mice
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Novdel3+/+ and Novdel3−/− male and female adult mice were fixed by perfusion with 4% paraformaldehyde (PFA) in PBS at 2, 6 and 12 months (n = 6 for each sex and genotype at each time point). The hind limbs were dissected and imaged by X-ray (KODAK In-Vivo Imaging System FX Pro) prior to decalcification in 10% EDTA pH 7. 7 μm coronal paraffin sections were prepared semi-serially.
3
Yeast-Encapsulated Antigen Delivery
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C57BL/6 mice received subcutaneous injections of YSL-OVA and LDH-OVA particles containing Cy5-OVA (20 μg OVA) in the inguinal region. The control group mice were injected with the same amount of Cy5-OVA alone. The fluorescence in the mice was detected at 0 h to 48 h to determine the distribution of YSL particles using the In vivo Imaging System FX Pro (KODAK, USA). To detect the trail of DCs and inflammation stimulated by YSLs, mice were sacrificed 4 days post-yeast administration. Draining lymph nodes were extracted and digested into a single cell suspension to detect the expression SIINFEKL-MHCI+ of recruited DCs. The antigen depots were homogenized to detect inflammation cytokines. Meanwhile, some were histologically evaluated with hematoxylin and eosin (H&E) staining to detect APC recruitment.
4
Radiographic Evaluation of Spinal Curvature
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X-ray of full-length radiographs was taken by In-Vivo Imaging System FX Pro (Kodak, USA) to evaluate the Cobb angle before injection and one week after injection. Rats in all groups were anesthetized by isoflurane and involved positioning them as straight as possible without any applied traction. Cobb angle at the primary curve in the coronal planes were measured by Digimizer software. The location of the upper or lower vertebra was together identified by two experienced evaluators of radiology. If there were different views, the average was selected.
5
Detecting IR-26-Specific Interacting Proteins
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Pull-down assay using IR-26–labeled AML cells were performed to detect IR-26–specific interaction proteins. Briefly, HL-60 cells were incubated with 1.25 μM IR-26 for 15 min. After washing with PBS, the cells were lysed to obtain the total protein. Then, the protein lysate was incubated with SDHA and ATP5B antibodies overnight at 4°C. A total of 100 μl of streptavidin-agarose (Santa Cruz Biotechnology) was added and incubated for 2 hours at room temperature, and the immunoglobulin G antibody was used as control. After the protein complex was centrifuged and washed, it was analyzed by SDS–polyacrylamide gel electrophoresis and imaging using Kodak In-Vivo Imaging System FX Pro to determine whether ATP5B or SDHA proteins were combined with the NIR fluorescence small-molecule IR-26 and then immunoblotted using anti-ATP5B or anti-SDHA antibodies to further verify the interactions.
6
Tumor Imaging with Radiolabeled RGD4CβL
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In the right flank (armpit region) of 6–7 week old BALB/c nude mice, 5×106 U87MG or A549 cells were implanted. The tumors were allowed to grow for 3 weeks subsequent to inoculation, then the animals received 7 µCi of the 125I-RGD4CβL, in 200 µl PBS, via the lateral tail vein under anesthesia. The images of the mice were taken using a small-animal imaging system (Kodak In-Vivo Imaging System Fx Pro, Kodak, Rochester, NY, USA) at several time points.
7
Characterization of Luminescent Phosphors
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Phase identification was performed by XRD (model SmartLab; Rigaku, Tokyo, Japan) operating at 40 kV/40 mA using nickel-filtered Cu Kα radiation and a scanning speed of 6.0° 2θ/min. Morphologies of the products were observed via TEM (model JEM-2000FX; JEOL, Tokyo). Photoluminescence of the phosphors was analyzed with an FP-8600 fluorospectrophotometer (JASCO, Tokyo). The persistent luminescence signals were obtained using Horiba JY FL3-21. The afterglow decay images were recorded in a dark room using a Kodak In-Vivo Imaging System FX Pro. The cell imaging was conducted by a laser scanning confocal microscope (LEICA TCS SP2, Germany).
All studies involving animals were approved by the university animal care and use committee.
All studies involving animals were approved by the university animal care and use committee.
8
In Vivo Biodistribution of Nanoparticles
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Male BALB/c nude mice were intravenously administrated with ICG-labeled NP-S1+Cur and CRT-NP-S1+Cur via the tail vein (ICG dose of 0.5 mg/kg), respectively. The fluorescent images were taken by the IVIS spectrum imaging system (Kodak In-Vivo Imaging System FX Pro, USA) at 0.5, 2, 6, 12, and 24 h post administration, and then the mice were sacrificed. The brains, hearts, livers, spleens, lungs and kidneys were washed with saline, and visualized under the In Vivo IVIS spectrum imaging system.
9
In Vivo Optical Imaging of Tumor Xenografts
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Athymic nude mice bearing tumor xenografts were injected intravenously with IR-26 at a dose of 0.3 mg/kg (n = 3). Whole body of mouse optical imaging was taken at day 2 after injection using Kodak In-Vivo Imaging System FX Pro (New Haven, CT) equipped with fluorescent filter sets (excitation/emission, 770/830 nm); all settings were applied as described previously (21 (link)). After mice were sacrificed, dissected organs and tumors were obtained for NIR fluorescent imaging at the day of sacrifice.
10
In Vivo NIR Imaging Analysis
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For in vivo imaging analysis, NIR dye IR-783 was loaded into SWCNTs-COOH and SWCNTs-HA-ss-DOX with the similar drug-loading content (~0.5%) according to the protocol above. When the tumor volume grew tô500 mm3, mice were injected with free IR-783 and IR-783-labeled SWCNTs samples at a dose of 2.5 mg/kg via the tail vein. NIR fluorescence imaging experiments were performed at 0.5, 1, 3, 6, and 8 hours postinjection using a Kodak in vivo imaging system FX PRO (Kodak, Rochester, NY, USA) equipped with an excitation band-pass filter at 750 nm and an emission at 783 nm. Images were analyzed using the Kodak Molecular Imaging Software 5.X.
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