Adult hermaphrodites were anesthetized with tricaine and tetramisole and immobilized between a coverslip and agarose pad on a slide. The time-lapse images shown in Figure 2A–E and Figure 4A–B were captured on an Olympus (Center Valley, PA) IX71 microscope equipped with a 60× PlanApo NA 1.42 oil objective and an Orca R2 CCD camera (Hamamatsu Photonics, Hamamatsu City, Japan). Hg arc excitation light was shuttered by a Sutter Lambda 10-3 shutter controller (Sutter Instruments, Novato, CA). Images shown in Figure 5A were captured with an Intelligent Imaging Innovations (Denver, CO) Marianas Spinning Disk Confocal equipped with a Photometrics (Tucson, AZ) Cascade QuantEM 512SC EMCCD, and Zeiss 63× 1.4 objective. Image sequences in Figure 6 were captured with a Perkin Elmer-Cetus (Waltham, MA) Ultraview Spinning Disk Confocal equipped with an Orca R2 CCD and an Olympus 60× 1.4 objective.
Sutter lambda 10 3 shutter controller
Manufactured by Sutter Instruments
The Sutter Lambda 10-3 is a shutter controller designed to precisely control the timing and operation of electromechanical shutters. It features three independent shutter channels, programmable shutter opening and closing times, and the ability to trigger the shutters with external signals or through computer control.
Automatically generated - may contain errors
Lab products found in correlation
Ix71 microscope, by Hamamatsu Photonics (1 mentions)
Orca r2 ccd camera, by Hamamatsu Photonics (1 mentions)
Orca r2 ccd, by Olympus (1 mentions)
1.4 na objective, by Olympus (1 mentions)
Na 1.4 objective, by Zeiss (1 mentions)
Ix81 microscope, by Olympus (1 mentions)
Orca flash 4.0 cmos camera, by Hamamatsu Photonics (1 mentions)
Quantem 512sc, by Zeiss (1 mentions)
2 protocols using sutter lambda 10 3 shutter controller
1
Live Imaging of Hermaphrodite Nematodes
2
High-Resolution Imaging of Cellular Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Images from Figures 1A , 2 , 5 , and 8 were captured on an Olympus IX81 microscope equipped with a 60× PlanApo numerical aperture (NA) 1.42 oil objective and an ORCA Flash 4.0 CMOS camera (Hamamatsu Photonics). Mercury arc excitation light was shuttered by a Sutter Lambda 10-3 shutter controller (Sutter Instruments), and an Olympus disk scanning unit was used for imaging fixed embryos or cells. Z-stacks were acquired with 200-nm steps.
Images inFigure 1B were captured on an Intelligent Imaging Innovations Marianas Spinning Disk Confocal equipped with a Photometrics Cascade QuantEM 512SC electron-multiplying charge-coupled device (CCD) and Zeiss 63×/NA 1.4 objective. Image sequences in Figures 3 , 4 , 6 , and 7 , Supplemental Figure S1, and supplemental videos were captured with a PerkinElmer-Cetus Ultraview Spinning Disk Confocal equipped with an Orca R2 CCD and an Olympus 60×/NA 1.4 objective. All images in Figures 1 and 2 were deconvolved using Huygens Professional X11 (SVI). Deconvolutions were performed using theoretical point spread functions calculated from the individual parameters for each microscope. Deconvolutions were run up to 40 iterations using CLME restoration.
Images in
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