All blood values recorded in this study, including CRP, bilirubin, albumin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and α-fetoprotein (AFP) levels and blood cell, neutrophil, lymphocyte, and platelet (PLT) counts, were determined within 5–7 days prior to treatment. Serum CRP levels were measured using latex-enhanced nephelometry (N-Latex CRP II; Siemens Healthcare Diagnostics, Tokyo, Japan). The LCR was calculated as follows: lymphocyte count (number/mL)/CRP level (mg/dL).21 The GPS,13 (link) modified GPS,14 (link) NLR,15 (link) PLR,16 (link) PI17 (link) and PNI18 (link) were constructed as described in Supplementary Table 1
N latex crp 2
Manufactured by Siemens
Sourced in Japan, Germany
The N-Latex CRP II is a laboratory diagnostic product manufactured by Siemens. It is a quantitative test used to determine the concentration of C-reactive protein (CRP) in human blood samples.
Automatically generated - may contain errors
Lab products found in correlation
Cobas e601 system, by Roche (2 mentions)
Liquitech creatinine pap 2, by Roche (2 mentions)
Human mmp 9 quantikine elisa kit dmp900, by R&D Systems (2 mentions)
L fabp, by Roche (1 mentions)
Llmipulse presto, by Fujirebio (1 mentions)
Vivid 7, by GE Healthcare (1 mentions)
Architect bnp jp, by Abbott (1 mentions)
Human tnf α immunoassay, by R&D Systems (1 mentions)
8 protocols using n latex crp 2
1
Biomarkers in Cancer Treatment Monitoring
2
Biomarkers Assessment in Acute Kidney Injury
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Immediately after admission, urinary and blood samples were collected in nonheparinized tubes and then centrifuged at 1000× g at 4 °C for 15 min, before storage at −80 °C until assayed. We measured the urinary L-FABP levels by an enzyme-linked immunosorbent assay (ELISA) using the Human L-FABP ELISA Kit (CMIC, Tokyo, Japan). Plasma B-type natriuretic peptide (BNP) levels were measured using a chemiluminesence enzyme immunoassay for human BNP (Shionogi & Co., Ltd., Osaka, Japan). We measured serum high-sensitivity troponin T (hs-TnT) levels via an electrochemiluminescence immunoassay, using a Cobas® e601 system (Roche Diagnostics, Tokyo, Japan), and serum high-sensitivity C-reactive protein (hs-CRP) levels via a latex-enhanced hs-CRP immunoassay (N-Latex CRP II, Siemens Healthineers, Tokyo, Japan). Serum creatinine levels were determined by an enzyme method, using the Liquitech® Creatinine PAP II (Roche Diagnostics, Tokyo, Japan) upon admission, daily until day 3, and then on day 7.
3
Plasma Biomarker Analysis of ACS and SAP
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In ACS (UAP and AMI) patients, blood samples were collected from an upper limb vein before emergent coronary angiography, and the blood was collected from the same vein on the second morning after admission in the SAP group. For plasma preparation, 2Na-EDTA was added to whole blood. After that, the blood sample was immediately centrifuged at 3000 rpm for 10 min, and the plasma was obtained and stored at –80°C until biomarker detection testing. The hs-CRP level in plasma was assayed according to the manufacturer's protocols (N-Latex CRP II; Siemens Healthcare Diagnostics, Malvern, PA, USA). The IL-6 was measured by commercially available ELISA kits, according to the protocols of the manufacturer (R&D Systems, Minneapolis, MN, USA).
4
Biomarker Measurement in Acute Kidney Injury
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Urinary and blood samples were collected in nonheparinized tubes immediately after admission and centrifuged at 1000 g at 4 °C for 15 min, before being stored at − 80 °C until assayed. Urinary L-FABP was measured by a latex turbidimetric immunoassay, using a Norudia® L-FABP (Sekisui Medical CO., Ltd., Tokyo, Japan), which had a lower detection limit of 1.5 ng/mL. Urinary L-FABP values below the lower detection limit of the assay were defined as 0.75 ng/mL. Serum NT-proBNP and high-sensitivity troponin T (hs-TnT) were measured using an electrochemiluminescence immunoassay and a Cobas® e601 system (Roche Diagnostics, Tokyo, Japan). Serum high-sensitivity C-reactive protein (hs-CRP) was measured using a latex-enhanced hsCRP immunoassay (N-Latex CRP II, Siemens Healthineers, Tokyo, Japan). Finally, the serum creatinine concentration was determined by an enzyme method, using the Liquitech® Creatinine PAP II (Roche Diagnostics, Tokyo, Japan) on admission, daily until day 3, and then on day 7.
5
Biomarker Profiling in Cardiac Disease
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Blood tests and two-dimensional echocardiography were performed upon study enrolment. To avoid the effect of exercise testing on the IL-6, blood sampling was performed following echocardiography in the supine position. Blood samples were collected from a peripheral vein into tubes containing aprotinin and ethylene diamine tetra acetic acid. The blood samples were centrifuged at 1000×g at 4 °C for 15 min to isolate serum or plasma and store it at − 80 °C until assayed. The plasma BNP concentration was measured using a chemiluminescence enzyme immunoassay for human BNP (LLMIPULSE Presto, Fujirebio Inc. Tokyo, Japan). The serum IL-6 concentration was measured using a chemiluminescence enzyme immunoassay for human IL-6 (human IL-6 measurement kit, Fujirebio Inc. Tokyo Japan) and serum high-sensitivity C-reactive protein (CRP) levels via a latex-enhanced hs-CRP immunoassay (N-Latex CRP II, Siemens Healthineers, Tokyo, Japan). A single echocardiographer who was blinded to the patients’ clinical information performed offline echocardiographic analysis using a Vivid 7 (GE Healthcare, Boston, Massachusetts, USA).
6
Biomarkers in Hemodialysis Patients
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All the patients were required to provide baseline plasma samples before HD session on the day underwent HD session, including hemoglobin, hemoglobin A1c, cholesterol profiles, BNP, high‐sensitive cTnT (hs‐cTnT), and high‐sensitive CRP (hs‐CRP). The samples were centrifuged within 60 minutes and stored at −80°C for further analysis. hs‐cTnT was measured by an electro chemiluminescence immunoassay (ECLIA) with the EClusys hs‐cTnT Roche diagnostic assay. In the hs‐cTnT assay, the 99th percentile of the upper reference limit and the lower limit of detection were 0.014 and 0.003 ng/mL, respectively. BNP was measured with a specific immunoradiometric assay for human BNP (ARCHITECT BNP‐JP; ABBOTT JAPAN Co, Ltd, Tokyo, Japan). The inter‐ and total coefficient variation for BNP was 1.1% to 5.1% and 2.3% to 5.3%, respectively. Hs‐CRP was measured using N‐latex CRP II (Dade Behring Inc., Marburg, Germany) with a coefficient variation of 3.1% at 0.5 mg/L.
7
Profiling Cardiovascular Biomarkers in PCI
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Peripheral blood samples were collected from all subjects on admission in preparation for planned percutaneous coronary intervention (PCI). Plasma samples were collected in ethylenediaminetetraacetic acid anticoagulant tubes and stored at −80 °C until assayed. Matrix metalloproteinase 9 (MMP-9) was analyzed with a commercially available kit (Human MMP-9 Quantikine ELISA Kit DMP900; R&D systems, Minneapolis, Minnesota, USA). Tumor necrosis factor-α (TNF-α) was analyzed with a commercially available kit (Human TNF-α immunoassay; R&D systems). High-sensitivity C-reactive protein (hs-CRP) was analyzed with a commercially available kit (N-Latex CRP II; Dade Behring, Marburg, Germany).
8
Plasma Biomarkers in Coronary Intervention
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Peripheral blood samples were collected from all subjects on admission in preparation for planned percutaneous coronary intervention. Plasma samples were collected in ethylenediaminetetraacetic acid anticoagulant tubes and stored at -80°C until assayed. Matrix metalloproteinase 9 (MMP-9) was analyzed with a commercially available kit (Human MMP-9 Quantikine ELISA Kit DMP900; R&D systems, Minneapolis, MN, USA). High-sensitivity C-reactive protein (hs-CRP) was analyzed with a commercially available kit (N-Latex CRP II; Dade Behring, Marburg, Germany).
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