Male and female mice aged 4–6 weeks were deeply anesthetized with ketamine and xylazine, head-fixed in a stereotax (Kopf Instruments), and injected via a small craniotomy over the region of interest, as described previously (Little and Carter, 2013 (link)). Viruses, fluorescently tagged Cholera Toxin Subunit B (CTB) (Life Technologies), or fluorescent retrobeads (Lumafluor) were pressure injected using a Nanoject II (Drummond) and left for 1–3 weeks prior to recording or perfusion for anatomy. All experimental procedures were approved by the University Animal Welfare Committee of New York University.
Cholera toxin subunit b ctb
Manufactured by Thermo Fisher Scientific
Cholera Toxin Subunit B (CTB) is a protein component of the cholera toxin. It functions as a binding subunit, facilitating the entry of the active subunit into target cells.
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Lab products found in correlation
2 protocols using cholera toxin subunit b ctb
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In Vivo Viral Injections and Tracing
2
In Vivo Viral Injections and Tracing
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Male and female mice aged 4–6 weeks were deeply anesthetized with ketamine and xylazine, head-fixed in a stereotax (Kopf Instruments), and injected via a small craniotomy over the region of interest, as described previously (Little and Carter, 2013 (link)). Viruses, fluorescently tagged Cholera Toxin Subunit B (CTB) (Life Technologies), or fluorescent retrobeads (Lumafluor) were pressure injected using a Nanoject II (Drummond) and left for 1–3 weeks prior to recording or perfusion for anatomy. All experimental procedures were approved by the University Animal Welfare Committee of New York University.
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