Recombinant human il 4

Manufactured by BioLegend

Recombinant human IL-4 is a cytokine that plays a key role in the regulation of immune and inflammatory responses. It is produced by activated T cells, mast cells, and basophils, and is involved in the differentiation of naive T cells into Th2 cells. Recombinant human IL-4 can be used for research purposes.

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Lab products found in correlation

Cd19 microbeads, by Miltenyi Biotec (1 mentions) Human ab serum, by Valley Biomedical (1 mentions) Anti human cd14 beads, by Miltenyi Biotec (1 mentions) Recombinant human gm csf, by BioLegend (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions)

Close spelling variants of this product

Recombinant IL-4 (12 citations) Human IL-4 (6 citations)

2 protocols using recombinant human il 4

Expansion of Human B Cells

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Autologous PBMCs were thawed, and CD19 Microbeads (Miltenyi Biotec, catalog 103-050-301) were used to isolate B cells. On day zero, 25,000 isolated B cells were seeded into 75 cm² cell culture flasks (Greiner Bio-One, catalog 658175) in the presence of irradiated (60 Gy) 3T3-CD40 ligand cells (obtained in-house) in complete RPMI supplemented with 5% Human AB Serum (Valley Biomedical, catalog HP1022) and 10 ng/mL recombinant human IL-4 (BioLegend, catalog 574004). On days 4 and 6, fresh medium (11 mL) plus IL-4 (10 ng/mL) was added to the flasks. On day 8, B cells were harvested and restimulated (780,000 B cells/flask) in the presence of irradiated 3T3-CD40 ligand cells and 10 ng/mL IL-4. On days 10 and 12, fresh medium (11 mL) plus IL-4 (10 ng/mL) were added. On day 13 or 14, expanded B cells were counted and cryopreserved until further analysis.

Duhen R., Fesneau O., Samson K.A., Frye A.K., Beymer M., Rajamanickam V., Ross D., Tran E., Bernard B., Weinberg A.D, & Duhen T. (2022). PD-1 and ICOS coexpression identifies tumor-reactive CD4+ T cells in human solid tumors. The Journal of Clinical Investigation, 132(12), e156821.

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Monocyte-derived DCs Activation by HGSOC IgGs

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PBMCs were isolated from the blood of 6 healthy volunteers by centrifugation with Ficoll-Paque Plus (GE Healthcare). CD14⁺ monocytes were purified using anti-human CD14 beads (Miltenyi) and cultured in RPMI 10% FCS, 20 ng/mL recombinant human GM-CSF and recombinant human IL4 (Biolegend). After 6 days, monocyte-derived DCs were re-stimulated with 10 μg/mLof IgGs (purified from protein extracts of two HGSOC omental tumors or healthy plasma IgGs and pooled) for 24 hours, pre-incubated or not with 30 mg of combined protein extracts from HGSOC cell lines G33 and AOCS1. Expression of the costimulation marker CD86 on DCs was assessed by flow cytometry.

Montfort A., Pearce O., Maniati E., Vincent B.G., Bixby L., Bӧhm S., Dowe T., Wilkes E.H., Chakravarty P., Thompson R., Topping J., Cutillas P.R., Lockley M., Serody J.S., Capasso M, & Balkwill F.R. (2016). A Strong B-cell Response Is Part of the Immune Landscape in Human High-Grade Serous Ovarian Metastases. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(1), 250-262.

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