Nanos gal4 vp16

Manufactured by Bloomington Drosophila Stock Center

Nanos-GAL4:VP16 is a genetic tool used in Drosophila research. It is a fusion protein that combines the DNA-binding domain of the GAL4 transcriptional activator with the VP16 transcriptional activation domain. This tool allows for the targeted expression of genes of interest in cells that express the Nanos gene, which is involved in germ cell development.

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Lab products found in correlation

Longgmr gal4, by Bloomington Drosophila Stock Center (1 mentions) Act5c gal4, by Bloomington Drosophila Stock Center (1 mentions) Tubp gal4, by Bloomington Drosophila Stock Center (1 mentions) Nanos gal4, by Bloomington Drosophila Stock Center (1 mentions) Aubhn2, by Bloomington Drosophila Stock Center (1 mentions) Aubqc42, by Bloomington Drosophila Stock Center (1 mentions) Spn e1, by Bloomington Drosophila Stock Center (1 mentions)

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Nanos-Gal4 (5 citations)

2 protocols using nanos gal4 vp16

Drosophila Genetic Toolkit Utilization

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The following fly stocks were obtained from Bloomington Stock Center: DJ667 GAL4 (stock #8171), Act5C-GAL4 (stock #4414), tubP-GAL4 (stock #5138), longGMR-GAL4 (stock 8605), nanos-GAL4:VP16 (stock #7312).

Jones T.I., Parilla M, & Jones P.L. (2016). Transgenic Drosophila for Investigating DUX4 and FRG1, Two Genes Associated with Facioscapulohumeral Muscular Dystrophy (FSHD). PLoS ONE, 11(3), e0150938.

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Drosophila Genetics: Fly Stock Maintenance

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All Drosophila stocks were maintained on cornmeal-molasses food between 23 and 25°C. qin¹, Df(3R)Exel6180, Df(2L)BSC187, aub^HN2, aub^QC42, ago3^t2, ago3^t3, spn-E¹, spn-E^Δ125, nanos-Gal4, nanos-Gal4::VP16, and tub>GFP were obtained from the Bloomington Stock Center. Qin::GFP was a gift from P. Zamore (University of Massachusetts Medical School, Worcester, MA). UASp-GFP::Aub was a gift from P. MacDonald (University of Texas, Austin, TX). qin^kumo was a gift from T. Kai (Temasek Research Laboratories, Singapore). The following trans-heterozygous allelic combinations were generated for all experimental analyses described: aub^HN2/aub^QC42 (aub), ago3^t2/ago3^t3 (ago3), spn-E¹/spn-E^Δ125 (spn-E), qin¹/Df(3R)Exel6180 (qin), and qin^kumo/Df(3R)Exel6180 (qin^kumo). Trans-heterozygous mutants were used to minimize the effects of second-site mutations on the relevant chromosomes.

Andress A., Bei Y., Fonslow B.R., Giri R., Wu Y., Yates JR I.I.I, & Carthew R.W. (2016). Spindle-E cycling between nuage and cytoplasm is controlled by Qin and PIWI proteins. The Journal of Cell Biology, 213(2), 201-211.

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