U2OS, A549, MCF10A, and MDA-MB-231 cells were obtained from ATCC and maintained at low passage numbers (generally less than 20 passages from the original vial). U2OS, A549, and MDA-MB-231 cells were grown in Dulbecco’s modified eagles medium (DMEM) supplemented with 10% FBS, 2 mM glutamine, and penicillin/streptomycin. MCF10A cells were grown in DMEM/F12 media, supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 mg/ml hydrocortisone, 100 ng/ml cholera toxin, 10 μg/ml insulin, and penicillin/streptomycin. Drugs were purchased from either Selleck Chemicals, APEXBio Technology, or Sigma-Aldrich. SYTOX green was purchased from Thermo-Fisher. Poly/Mono ADP Ribose Rabbit mAb (83732) was purchased from Cell Signaling Technologies. Purified rabbit anti-active caspase 3 (559565) and Alexa Fluor® 647 conjugated mouse anti-cleaved PARP (558710) were purchased from BD Pharmingen. Secondary antibodies were purchased from LICOR Biosciences and BD Biosciences. See also Supplementary Table 2 .
Alexa fluor 647 conjugated mouse anti cleaved parp 558710
Manufactured by BD
Alexa Fluor® 647 conjugated mouse anti-cleaved PARP (558710) is a laboratory product that binds to the cleaved form of poly(ADP-ribose) polymerase (PARP), a protein involved in cellular processes. The product consists of a mouse monoclonal antibody labeled with the fluorescent dye Alexa Fluor® 647, which can be used for the detection and analysis of cleaved PARP in various experimental applications.
Automatically generated - may contain errors
2 protocols using alexa fluor 647 conjugated mouse anti cleaved parp 558710
1
Cell Culture and Reagent Preparation for Assays
2
Cell Culture and Reagent Preparation for Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
U2OS, A549, MCF10A, and MDA-MB-231 cells were obtained from ATCC and maintained at low passage numbers (generally less than 20 passages from the original vial). U2OS, A549, and MDA-MB-231 cells were grown in Dulbecco’s modified eagles medium (DMEM) supplemented with 10% FBS, 2 mM glutamine, and penicillin/streptomycin. MCF10A cells were grown in DMEM/F12 media, supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 mg/ml hydrocortisone, 100 ng/ml cholera toxin, 10 μg/ml insulin, and penicillin/streptomycin. Drugs were purchased from either Selleck Chemicals, APEXBio Technology, or Sigma-Aldrich. SYTOX green was purchased from Thermo-Fisher. Poly/Mono ADP Ribose Rabbit mAb (83732) was purchased from Cell Signaling Technologies. Purified rabbit anti-active caspase 3 (559565) and Alexa Fluor® 647 conjugated mouse anti-cleaved PARP (558710) were purchased from BD Pharmingen. Secondary antibodies were purchased from LICOR Biosciences and BD Biosciences. See also Supplementary Table 2 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!