α chemotrypsin

Manufactured by Merck Group

α-chemotrypsin is a serine protease enzyme used in various laboratory applications. It functions by cleaving peptide bonds on the carboxyl side of aromatic amino acid residues. The enzyme is extracted and purified from bovine pancreas.

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Lab products found in correlation

Reduced glutathione, by Merck Group (2 mentions) Dithiothreitol (dtt), by Merck Group (2 mentions) Isopropyl β d 1 thiogalactopyranoside (iptg), by Merck Group (2 mentions) Lithium chloride (licl), by Merck Group (2 mentions) Imidazole, by Merck Group (2 mentions) N succinyl ala ala pro phe p nitroanilide, by Merck Group (2 mentions) Trifluoroethanol, by Merck Group (2 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) 8 anilino 1 naphthalenesulfonic acid, by Merck Group (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions) Polymyxin b, by Merck Group (1 mentions)

2 protocols using α chemotrypsin

Protein Purification and Characterization

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IPTG, imidazole, N-succinyl- Ala-Ala-Pro-Phe-p-nitroanilide, trifluoroethanol, LiCl, α-chemotrypsin, 8-anilino-1-naphthalene-sulfonic acid (ANS), reduced Glutathione, DTT and MTT were obtained from Sigma. All cell culture reagents were obtained from GIBCO. All enzymes were purchased from NEB (USA); ELISA kit from Peprotech and toxicity removal kit from Norgen. All reagents used were analytical grade. The plasmids and strains used in this study are listed (S1 Supporting Information).

Pandey S., Sharma A., Tripathi D., Kumar A., Khubaib M., Bhuwan M., Chaudhuri T.K., Hasnain S.E, & Ehtesham N.Z. (2016). Mycobacterium tuberculosis Peptidyl-Prolyl Isomerases Also Exhibit Chaperone like Activity In-Vitro and In-Vivo. PLoS ONE, 11(3), e0150288.

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Preparatory Reagents for Biochemical Assays

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IPTG, imidazole, α-chemotrypsin, reduced Glutathione, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, trifluoroethanol, LiCl, DTT, H₂O₂, and polymyxin B were purchased from Sigma. Cell culture reagents were purchased from GIBCO, Thermo Fisher Scientific (USA). All restriction enzymes were purchased from New England Biolabs (USA); antibodies from Abcam (UK); and ELISA kits from Peprotech (USA). Mycobacterium growth media and supplements were purchased from Becton, Dickinson and Company (USA). All reagents used were analytical grade. The strains and plasmids used in this study are listed in Table S1.

Pandey S., Tripathi D., Khubaib M., Kumar A., Sheikh J.A., Sumanlatha G., Ehtesham N.Z, & Hasnain S.E. (2017). Mycobacterium tuberculosis Peptidyl-Prolyl Isomerases Are Immunogenic, Alter Cytokine Profile and Aid in Intracellular Survival. Frontiers in Cellular and Infection Microbiology, 7, 38.

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