Lactate dehydrogenase ldh kit

Human macrophages were obtained from the Chinese Academy of Sciences (China). Amygdalin (≥ 98% by HPLC, CAS No.: 29883–15–6) was purchased from Chengdu Alfa Biotechnology Co., Ltd. (China). SYBR Premix Ex Taq II Reagent Kit was purchased from Toyobo (China). IL-18, IL-1β, and IL-6 were obtained from Enzyme Marker Biology (China). A Cell Counting Kit-8 (CCK-8) and Hoechst 33258 staining solution were purchased from Beyotime Biotechnology Co., Ltd. (China). A lactate dehydrogenase (LDH) kit, FITC Annexin V apoptosis detection kit, and phosphate-buffered saline (PBS) powder were purchased from Beyotime Biotechnology Co., Ltd.

Rat adrenal pheochromocytoma cells (PC12 cells; highly differentiated type) were purchased from the Cell Center of Shanghai Academy of Life Sciences, Chinese Academy of Sciences. Aβ_25-35 (purity ≥ 95%, no. Y0044) was from Beijing Biosynthesis Biotechnology Co., Ltd. (China). DMEM, streptomycin, trypsin, and fetal bovine serum were from HyClone Laboratories Inc. (Logan, Utah, USA). MTT was from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). A lactate dehydrogenase (LDH) kit was purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO), and glutathione peroxidase (GSH-Px), as well as catalase (CAT), total antioxidant capacity (T-AOC), and acetylcholinesterase (AChE) kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The primary antibodies against sirtuin1 (Cat. No. 13161-1-AP), total Nrf2 (Cat. No. 16396-1-AP), nuclear Nrf2 (Cat. No. 66504-1-Ig), HO-1 (Cat. No. 66743-1-Ig), β-actin (Cat. No. 60008-1-Ig), and PCNA (Cat. No. 60097-1-Ig) were from Proteintech Co., Ltd. (Chicago, USA). HRP-labeled goat anti-mouse IgG antibody was purchased from Santa Cruz Biotechnology, Inc. (Cat. No. sc-2005; Santa Cruz, California, USA). HRP-labeled goat-rabbit IgG antibody was from Proteintech Co., Ltd. (Cat. No. 10285-1-AP; Chicago, USA).

Manganese (II) chloride tetrahydrate, BDNF, and DuoLink proximity ligation assay (PLA) reagent were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The pGCSIL-GFP-α-Syn small hairpin RNA (shRNA)^{10 (link)} was purchased from Shanghai GeneChem Co. Ltd. (Shanghai, China). A Cell Counting Kit-8 (CCK-8) assay kit and lactate dehydrogenase (LDH) kit were purchased from Beyotime Biotech Co. Ltd. (China). α-Syn primary antibody was obtained from Thermo Fisher Scientific. PSD95, SynGAP, β-actin, phospho-CaMKII (Thr286), CaMKII-α, NMDA receptor 2A (GluN2A), NMDA receptor 2B (GluN2B), Fyn, phospho-NMDA receptor 2A (GluN2A) (Tyr1246), and phospho-NMDA receptor 2B (GluN2B) (Tyr1472) primary antibodies were purchased from Cell Signaling Technology. TrkB, AKT, and AKT-phospho-S473 primary antibodies were purchased from Proteintech Biotech Co. Ltd. (China). BDNF and phospho-Fyn (Y530) were purchased from Abcam Ltd. (Hong Kong). Secondary antibodies including horseradish peroxidase (HRP)-conjugated anti-rabbit and HRP-conjugated anti-mouse were purchased from Abcam Ltd. (Hong Kong).

Human kidney proximal tubular epithelial (HK-2) cells were purchased from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). Dulbecco’s modified Eagle’s medium (DMEM) and Fetal bovine serum were purchased from HyClone Biochemical Products Co., Ltd. (UT, USA). Penicillin and streptomycin were purchased from Beijing Pubo Biotechnology Co., Ltd. (Beijing, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). Lactate dehydrogenase (LDH) kit, hematoxylin–eosin (H&E) dye, 2′,7′-dichlorofluorescein diacetate dye (DCFH-DA) and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1), Annexin V-FITC, Propidium iodide (PI), and Rabbit anti-mouse IgG conjugated with fluorescein isothiocyanate (FITC-IgG) were all purchased from Shanghai Beyotime Bio-Tech Co., Ltd. (Shanghai, China). Cell culture plates were purchased from Wuxi Nest Bio-Tech Co., Ltd. (Wuxi, China). Calcium chloride (CaCl₂), sodium oxalate (Na₂Ox), ethylenediaminetetraacetic acid (Na₂EDTA), dodecyl sodium sulfate (SDS) and the other conventional reagents were all analytically pure and purchased from Guangzhou Chemical Reagent Factory of China (Guangzhou, China).