Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from Invitrogen-Gibco (Grand Island, NY, USA). ELISA kits for PGE2, TNF-α, IL-1ß, and IL-6 were purchased from R & D Systems, Inc. (St. Louis, MO, USA) and BD Biosciences (San Diego, CA, USA). Anti-JNK, anti-phosphorylated JNK (anti-p-JNK), anti-ERK1/2, anti-phosphorylated ERK1/2 (anti-p-ERK1/2), anti-p38, and anti-phosphorylated p38 (anti-p-p38) mouse or rabbit antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Pyrrolidine dithiocarbamate (PDTC), a specific inhibitor of NF-κB, was purchased from Calbiochem (San Diego, CA, USA). All other reagents were purchased from Sigma-Aldrich Chemical Co. (St Louis, MO, USA).
Anti phosphorylated p38 anti p p38
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-phosphorylated p38 (anti-p-p38) is a laboratory reagent used to detect and quantify the phosphorylated form of the p38 mitogen-activated protein kinase (MAPK). p38 MAPK is a key signaling molecule involved in various cellular processes, including stress response, inflammation, and cell differentiation. The anti-p-p38 antibody specifically binds to the phosphorylated form of p38 MAPK, enabling researchers to analyze its activation and signaling dynamics within cells or tissues.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Anti erk1 2, by Cell Signaling Technology (3 mentions)
Anti phosphorylated jnk anti p jnk, by Cell Signaling Technology (3 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Ripa lysis buffer, by Merck Group (2 mentions)
Anti p38, by Cell Signaling Technology (2 mentions)
Enzyme linked immunosorbent assay elisa kits for the analyses of tnf α, by BD (2 mentions)
Anti phosphorylated erk1 2 anti p erk1 2, by Cell Signaling Technology (2 mentions)
Lipopolysaccharides (lps), by Merck Group (2 mentions)
Anti jnk, by Cell Signaling Technology (2 mentions)
Recombinant human tnf α, by BioLegend (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Ifn γ, by BioLegend (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Anti p65, by Cell Signaling Technology (1 mentions)
Elisa kits for tnf α, by R&D Systems (1 mentions)
4 protocols using anti phosphorylated p38 anti p p38
1
Inflammatory Response Signaling Pathways
2
LPS-Induced Inflammatory Response Pathway
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LPS, phosphate buffered saline (PBS), dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and radio-immunoprecipitation assay RIPA lysis buffer were bought from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM) were purchased from Invitrogen-Gibco (Grand Island, NY, USA). Enzyme-linked immunosorbent assay (ELISA) kits for the analyses of TNF-α, IL-6, and PGE2 were obtained from BD Biosciences (San Diego, CA, USA) and R&D Systems, Inc. (St. Louis, MO, USA). Antiphosphorylated IκB-α (anti-p-IκB-α), anti-NF-κB, anti-JNK, anti-p38, anti-ERK1/2, anti-phosphorylated JNK (anti-p-JNK), anti-phosphorylated p38 (anti-p-p38) and anti-phosphorylated ERK1/2 (anti-p-ERK1/2) mouse or rabbit antibodies were bought from Cell Signaling Technology (Beverly, MA, USA). All other reagents were obtained from Sigma-Aldrich.
3
Pro-inflammatory Signaling Pathway Investigation
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LPS, phosphate-buffered saline (PBS), dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and RIPA lysis buffer were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle medium (DMEM) were purchased from Invitrogen (Grand Island, NY, USA). Recombinant human TNF-α and IFN-γ were purchased from BioLegend (San Diego, CA, USA). Enzyme-linked immunosorbent assay (ELISA) kits for the analyses of TNF-α, IL-6, and PGE2 were obtained from BD Biosciences (San Diego, CA, USA) and R&D Systems, Inc. (St. Louis, MO, USA). Antiphosphorylated IκB-α (anti-p-IκB-α), anti-NF-κB, anti-JNK, anti-p38, anti-ERK1/2, antiphosphorylated JNK (anti-p-JNK), antiphosphorylated p38 (anti-p-p38), and antiphosphorylated ERK1/2 (anti-p-ERK1/2) mouse or rabbit antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA) or Santa Cruz Biotechnology Inc. (Dallas, TX, USA). All other reagents were obtained from Sigma-Aldrich.
4
Anti-inflammatory Mechanisms of Compound X
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All chemicals were commercially available of analytical purity (Jiangsu Hanbang Science and Technology, Co., Ltd., Huai’an, China). Trypsin, streptomycin, penicillin, MTT, dimethyl sulfoxide (DMSO) and LPS were obtained from Sigma-Aldrich Chemical Co., Ltd. (St. Louis, MO, USA). Dulbecco’s modified Eagle medium (DMEM, high-glucose) was purchased from Hyclone Lab (Logan, UT, USA). Fetal bovine serum (FBS) was brought from Zhejiang Tianhang Biological Technology Co., Ltd. (Hangzhou, China). ELISA kits for TNF-α, PGE2, IL-1β and IL-6 were purchased from R&D Systems, Inc. (St. Louis, MO, USA). Anti-GAPDH, anti-PCNA (proliferating cell nuclear antigen), anti-iNOS, anti-COX-2, anti-JNK, anti-phosphorylated JNK (anti-p-JNK), anti-ERK1/2, anti-phosphorylated ERK1/2 (anti-p-ERK1/2), anti-p38, anti-phosphorylated p38 (anti-p-p38), anti-p65, anti-IκB-α, anti-phosphorylated-IκB-α (anti-p-IκB-α) mouse or rabbit antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).
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