For this study, we have used bovine incisors, which were obtained from a local slaughterhouse (VION, Bad Bramstedt, Germany, vionfoodgroup.com ). The samples had originally not been collected for the purpose of research, but were obtained after slaughtering as described in previous publications, without ethical approval being seeked [12] (link). Extraction of incisors was performed under supervision of the local veterinary, and teeth were only utilized after being declared free of infectious diseases. From each of 32 bovine incisors of the second dentition, 4 axial root slices (5 mm height) were cut (Band Saw Exakt 300cl, Exakt Apparatebau, Norderstedt, Germany) and embedded in acrylic resin (Technovit 4071, Heraeus Kulzer, Hanau, Germany). Embedded slices were then ground flat into cubicles (Phoenix Alpha; Buehler, Düsseldorf, Germany), plan-parallelised, and polished (abrasive paper 1200, 2400, 4000 Exakt Apparatebau) under water cooling. Two thirds of the resulting exposed dentin surface (3×5 mm) were covered with a tape, and the complete dentin surface was covered with acid-resistant nail varnish (Maybelline, New York, USA). The tape was then carefully removed with a scalpel, resulting in a defined experimental area, which was then controlled using a stereomicroscope (Stemi, Zeiss, Jena, Germany).
Stemi
Manufactured by Zeiss
Sourced in Germany
The Stemi is a stereomicroscope designed and manufactured by Zeiss. It provides a three-dimensional, high-resolution image of the observed specimen. The Stemi features an optical system that allows for continuous zoom and variable magnification, enabling users to examine samples in detail.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using stemi
1
Bovine Incisor Preparation for Dentin Surface Study
2
Microscopic Examination of Plant Anatomy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zeiss AxioLab trinocular microscope and Zeiss Stemi stereo microscope were used to capture the photomicrographs using different magnifications. For studying the intercellular components like crystals, starch grains and lignified walls polarized light and for histological study bright field light microscope were used. Descriptive anatomical terms provided by Metcalfe, 1964 , Fahn, 1987 were used.
3
Visualizing Brain Samples with Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Visualization of stained brain sections or cells was performed using a Stemi stereomicroscope or an AxioImager (Zeiss) fitted with an AxioCam MRc5 digital camera, or using a TCS SP5 confocal microscope (Leica). Images were captured using the AxioVision (Zeiss) or the integrated LAC (Leica) software. Confocal images were assembled using LAS AF lite (Leica Application Suite, Leica) or Image J (developed by Wayne Rasband, National Institutes of Health; http://imagej.nih.gov/ij ). All images were processed with Image J and/or Adobe Photoshop.
4
Macroinvertebrate Taxonomic Identification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Macroinvertebrates were identified under a stereomicroscope (Zeiss Stemi, Deutschland). Identification was made to the lowest possible taxonomic level using identification keys (e.g. Tachet et al. 2000 , Oscoz et al. 2011 (link), Kriska 2013 (link)). Nonetheless, to facilitate understanding of results, Poduromorpha Entomobryomorpha Symphypleona Collembola Isopoda Podocopida Copepoda Crustacea Pulmonata Sphaeriida Mollusca Sarcoptiformes Trombidiformes Acari Tricladida Platyhelminthes Haplotaxida Lumbriculida Enchytraeida Arhynchobdellida Annelida
5
Metastatic Spread and Necrosis Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To compare the level of metastasis and associated necrosis between biopsied and non-biopsied mice, a total of 84 mice in four cohorts underwent the metastasis model described above. Cohort size was designed to allow manageable numbers of mice and enable consistent treatment and surgical handling. Seven days after the tumor excision, each cohort of mice consisting, on average, of biopsy (n = 10) and non-biopsy (n = 11) mice was sacrificed. The draining lymph nodes and lungs were harvested, formalin fixed, and paraffin embedded with each block assigned an anonymizing numerical identifier to allow unbiased evaluation. The anonymized lungs, lymph nodes, and tumors were serial sectioned into 5-μm thin tissue sections and stained with hematoxylin and eosin (H&E) for histologic evaluation. Imaging was performed using a Zeiss Axiocam HRC Color, mounted on a Zeiss Stemi. The percentage of metastatic (lung and lymph node) and necrotic (tumor) tissue areas relative to total section area was established using an overlay grid imposed on images captured from H&E-stained tissue sections.
6
Detailed Morphological Analysis of Gloeocystidiellum sp.
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Only Gloeocystidiellum sp., after positive antibacterial activity, was analyzed morphologically in detail. In order to perform the analysis, freehand sections were made with a razor blade under a stereomicroscope (Stemi Carl Zeiss). The microscopic procedure followed Xing et al. [49 (link)]. The preparations of the sections were with phloxine 1% and decolorization with 10% potassium hydroxide (KOH) solution and Congo Red 1%. The amyloid reaction was evaluated with Melzer’s reagent (Figure 2 ). A sulfoaldehyde (SA) reaction to detect a sulfuric reaction of gloecystidia was performed with sulfuric acid + vanillin (Sigma-Aldrich). Observations were under a light microscope (CX31, Olympus) at 100× magnification. A detailed illustration (Figure 3 ) of the specimen was performed by hand using a scale (1 × 1 cm2 = 5 × 5 µm2) and later revision of the taxonomic key for the genus available in Wu, Larsson, and Ryvarden [50 ,51 ]. Color codes are based on the online server https://encycolorpedia.es/ (11 July 2022) [52 ].
7
Clonogenic Survival Assay Protocol
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!