M1 macrophage generation medium dxf
M1-Macrophage Generation Medium DXF is a cell culture medium designed for the differentiation and expansion of M1-polarized macrophages. It provides the necessary components to support the in vitro generation of M1-type macrophages.
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6 protocols using m1 macrophage generation medium dxf
Differentiation of M1 Macrophages from PBMCs
Monocyte-derived M1 Macrophage Generation
Primary Human M1 Macrophage Transport
Cell Culture and Differentiation Protocols for Macrophage Studies
Influenza A Virus Infection Model
Human monocyte-derived macrophages (hMDM) were isolated from healthy donors by using Histopaque (Sigma Aldrich) and separated by density gradient centrifugation, plated in monocyte attachment medium (Promocell, Heidelberg, Germany) for 2 h and cultivated in M1-Macrophage Generation Medium DXF (Promocell) for 7 d.
Madin-Darby canine kidney (MDCK) cells were cultured in Eagle minimum essential medium (EMEM, Sigma Aldrich) with 10 % FBS and 1 % P/S.
In vitro infections were performed using influenza A virus/IAV/H1N1/Puerto Rico/8/1934 (PR8). For the in vivo model the mouse-adapted strain influenza A virus/IAV/H1N1/vi0084/2016 was used. Ex vivo infections utilized the mouse-adapted H1N1 influenza A virus strain HA-G222-mpJena/5258 [15 (link)]. To identify plaque-forming units (PFU), standard plaque assay was performed as described previously [16 (link)].
Macrophage Phenotypic Polarization Protocol
A human macrophage cell line was obtained from PromoCell GmbH (Heidelberg, Germany) and cultured in Monocyte Attachment Medium (PromoCell GmbH). In addition, for their differentiation into M1- and M2-like macrophages, cells were cultured in M1-Macrophage Generation Medium DXF (Promo-Cell GmbH) and M2-Macrophage Generation Medium DXF (PromoCell GmbH), respectively.
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