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Hmgb1

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HMGB1 is a high mobility group box (HMGB) protein that functions as a nuclear DNA-binding protein. It plays a role in the organization of DNA and regulation of gene expression. HMGB1 is involved in various cellular processes, including DNA repair, inflammation, and cell signaling.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (6 mentions) Sybr green, by Thermo Fisher Scientific (3 mentions) A3500, by Promega (3 mentions) Gapdh, by Qiagen (2 mentions)

Close spelling variants of this product

HMGB1 siRNA (1 citations)

3 protocols using hmgb1

1

Quantifying Gene Expression in Bladder Tissue

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Total RNA was extracted from wild type and MIF knockout mouse bladder tissue through Trizol (15596026, ThermoFisher Scientific, Grand Island, NY), DNA removed by DNase, and reversed transcribed to cDNA (A3500, Promega, Madison, WI). SYBR green (4472903, ThermoFisher Scientific, Grand Island, NY) was utilized with primers (HMGB1, PPM05059F; Rn18s, PPM72041A, Qiagen, Germantown, MD) to quantified level of mRNA in bladder tissue from wild type and MIF knockout mice. 18S rRNA was used as the internal control.
Ma F., Kouzoukas D.E., Meyer‐Siegler K.L., Hunt D.E., Leng L., Bucala R, & Vera P.L. (2017). Macrophage migration inhibitory factor mediates protease‐activated receptor 4‐induced bladder pain through urothelial high mobility group box 1. Physiological Reports, 5(24), e13549.
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2

Quantifying MIF and HMGB1 mRNA in Bladder

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Total RNA was extracted from wild-type and MIF knockout mouse bladder tissue through Trizol (15596026; Thermo-Fisher Scientific, Grand Island, NY, USA), DNA removed by DNase and reversed transcribed to cDNA (A3500; Promega, Madison, WI, USA). PCR products were detected using SYBR green (4472903; ThermoFisher Scientific) binding with primers (MIF, PPM02985H; HMGB1, PPM05059F; GAPDH, PPM02946E; Qiagen, Germantown, MD, USA) to quantify the level of mRNA in bladder tissue from wild-type and MIF knockout mice. The detection of GAPDH PCR product was used as the internal control.
Ma F., Hunt D.E., Leng L., Bucala R., Meyer-Siegler K.L, & Vera P.L. (2018). Protease activated-receptor 4 activation as a model of persistent bladder pain: Essential role of macrophage migration inhibitory factor and high mobility group box 1. International journal of urology : official journal of the Japanese Urological Association, 25(10), 887-893.
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3

Quantifying Spinal mRNA Expression

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Total RNA was extracted from L6-S1 spinal cord of PAR4- or scramble-treated mice through Trizol (15596026, ThermoFisher Scientific, Grand Island, NY), DNA removed by DNase, and reversed transcribed to cDNA (A3500, Promega, Madison, WI). PCR products were detected using SYBR green (4472903, ThermoFisher Scientific, Grand Island, NY) binding with primers (MIF, PPM02985H; HMGB1, PPM05059F; GAPDH, PPM02946E, Qiagen, Germantown, MD) to quantify levels of mRNA in spinal tissue from two groups of mice. Detection of GAPDH PCR product was used as the internal control.
Ma F., Meyer-Siegler K.L., Leng L., Bucala R, & Vera P.L. (2019). Spinal macrophage migration inhibitory factor and high mobility group box 1 mediate persistent bladder pain. Neuroscience letters, 699, 54-58.
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