Four strawberry fruits for each replicate with three biological replicates for each time point were treated with the following hormonal compounds: 1 mM ABA (Sigma), 1 mM 1-NAA (auxin; Sigma), 10 μM 24-epibrassinolide (brassinosteroid; Sigma), 2 g/L Ethrel (ethylene, Bayer CropScience) or 100 μM methyl jasmonate (Sigma). All compounds were dissolved in ethanol, and the corresponding buffer-only controls were also performed. Fruits at the C2 developmental stage were placed in a plastic box and immersed in citrate buffer containing each hormone, and then collected at different time points post-treatment. All samples were frozen in liquid nitrogen and stored at -80°C.
Ethrel
Manufactured by Bayer
Sourced in Israel
Ethrel is a plant growth regulator manufactured by Bayer. It is a liquid formulation containing the active ingredient Ethephon, which releases ethylene within plants. Ethrel is used to promote various physiological responses in plants.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using ethrel
1
Strawberry Hormone Treatments Dynamics
2
Ethephon and Antioxidant Treatment for Fruit Ripening
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fruiting branches on the tree were dipped in a 2-L container filled with an ethephon agent (pH = 5.5) containing 4% monopotassium phosphate (MPK; Haifa NPK, Haifa, Israel), 0.2% ethrel (Bayer Cropscience, Monheim, Germany) and 0.3% paraffin oil (Drexel, Memphis, TN, USA). The antioxidant treatments were applied by adding either 0.3% ascorbic acid (Galvit C, Biovac, Or Akiva, Israel) or 100 mM butyric acid (Sigma-Aldrich, St. Louis, MO) to the ethephon agent. Control branches were similarly dipped in a 2-L water container. All DF measurements during the experiment were carried out on leaves and fruits while attached to the tree.
3
Nodulation Phenotyping of Aeschynomene Mutants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, A. evenia CIAT22838 was used as a WT reference plant and in some experiments its ccamk-3 mutant as negative control (Quilbé et al. 2021 (link); Quilbé, Nouwen, et al. 2022 (link)). All analyzed orm mutants were isolated from the EMS-mutagenized collection of A. evenia CIAT22838 plants that have been shown to have phenotypic defects in nodulation (Quilbé et al. 2021 (link)). A. evenia seeds were scarified for 40 min with sulfuric acid (96%) and rinsed several times with distilled water; whereafter, germination was induced by overnight incubation in distilled water containing 0.01% (v/v) ethrel (BAYER). For root phenotyping and seed production, 1-d-old seedlings were transferred to plastic pots filled with attapulgite hereafter the plants were cultivated in the greenhouse (28 °C and 70% relative humidity) as detailed in Quilbé et al. (2021) (link).
4
Ethephon-Induced Rubber Tree Transcriptome
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pará rubber tree (H. brasiliensis clone PB260) seeds were collected in the field of PT. Bridgestone Sumatra Rubber Estate, Serbalawan, North Sumatra, Indonesia. These seeds were germinated and the seedlings cultivated in the experimental greenhouse of the Agency for the Assessment and Application of Technology (BPPT), Serpong, Tangerang Selatan, Indonesia. Approximately 6-week-old seedlings, which were about 50 cm in height, were used for experiments. The stem region 2–5 cm below the shoot apex was swabbed with either 2.5% Ethrel® (containing 24% ethephon; Bayer CropScience, Inc.) or distilled water (mock control) using small brushes at around 09:00 in the greenhouse. At three time points (6, 24, and 48 h) after treatment, the treated stem region (3 cm long) was collected and placed in small plastic bags, immediately frozen in liquid nitrogen, and stored at − 80 °C until used for RNA extraction.
5
Mutant Characterization and Seed Germination in A. evenia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All A. evenia nodulation mutants characterized in this study were obtained from the phenotypic screen of an EMS-mutagenized population derivated from the reference CIAT22838 line (Quilbé et al., 2021 (link)). Mutant characteristics are detailed in Supplemental Table S1 . For the A21 and E26 mutants, genetic analyses (genetic determinism and allelism tests) and sequencing approaches (genotyping and mapping-by-sequencing) were performed as indicated in Quilbé et al. (2021) (link).
For seed germination, seeds were scarified for 40 min with sulfuric acid (96% v/v) and rinsed with distilled water; germination was induced over night with 0.01% (v/v) ethrel (BAYER) as described earlier (Chaintreuil et al., 2016 (link)).
For seed germination, seeds were scarified for 40 min with sulfuric acid (96% v/v) and rinsed with distilled water; germination was induced over night with 0.01% (v/v) ethrel (BAYER) as described earlier (Chaintreuil et al., 2016 (link)).
6
Ethephon-Based Fruit Thinning in Apples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experiment 1: three different rates (75, 150, 300 mg L -1 ) of ethephon (2chloroethyl-phosphonic acid, Ethrel, Bayer CropScience Inc) were tested at five different stages, specifically at 30 % full bloom (FB), 100 % FB, 30 days after full bloom (DAFB), 40 DAFB and 50 DAFB. Ethephon treatments were compared to a hand-thinning treatment and an untreated control (UTC). Experiment 2: after considering the results of the previous experiment, the three ethephon rates were tested at two stages, close to 100 % FB and 40 DAFB. Experiment 3: the three ethephon rates were tested again at 100 % FB and 40 DAFB and, in addition, two treatments of ethephon 150 mg L -1 at 30 % FB and 50 DAFB, respectively, were added to validate the results obtained in experiment 1.
All ethephon treatments were sprayed very early in the morning, when air temperatures were below 25 °C. A high-pressure handgun sprayer (25 atm) was used at a rate of ~1000 L ha -1 . Hand-thinning was carried out at 45-55 DAFB by spacing fruit ~15-20 cm apart. The application time for each treatment and year and the evolution of temperatures is presented in Figure 1.
All ethephon treatments were sprayed very early in the morning, when air temperatures were below 25 °C. A high-pressure handgun sprayer (25 atm) was used at a rate of ~1000 L ha -1 . Hand-thinning was carried out at 45-55 DAFB by spacing fruit ~15-20 cm apart. The application time for each treatment and year and the evolution of temperatures is presented in Figure 1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!