Szx12 dissecting microscope

Manufactured by Olympus

Sourced in Canada

The SZX12 dissecting microscope is a high-quality optical instrument designed for precise observation and inspection. It features a zoom range of 6.3x to 80x, providing users with a clear and detailed view of their specimens. The microscope's ergonomic design and user-friendly controls make it a reliable tool for a variety of applications.

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17 protocols using szx12 dissecting microscope

Genitalia Extraction and Measurement Protocol

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All specimens studied were adults. Genitalia extractions and preservation largely followed the methodology described by Smith (1979) . Specimens were examined using Wild M5A and Olympus SZ61 stereoscopes. Measurements were made using an ocular scale in an Olympus WHSZ10X-H/22 eyepiece on an Olympus SZ61 stereoscopic microscope. Microphotography employed an Olympus SzX12 dissecting microscope equipped with an MTI 3CCD camera and an Olympus MVX10 dissecting microscope equipped with an Olympus DP70 camera. Image montage employed Olympus cellSens software. Images were processed with Adobe Photoshop 2020 (21.1.2). Descriptive terminology largely follows Konstantinov (1998) .
Specimens are deposited in the Monte L. Bean Life Science Museum, Brigham Young University (Provo, Utah, U.S.A.; BYUC), Museo de Zoología de la Pontificia Universidad Católica del Ecuador (Quito, Ecuador; QCAZ), and Travancore Insect Collection, Kerala Agricultural University (Vellayani, Kerala, India; TIC). Label data from the specimens are presented verbatim; a backwards slash (\) indicates a new line on a label.

Viswajyothi K, & Clark S.M. (2020). Prathapanius fortis, a new genus and new species of Galerucinae from Ecuador (Coleoptera, Chrysomelidae). ZooKeys, 968, 111-126.

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Characterization of Fynbos Fungal Strains

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Fynbos strains were characterised on Czapek yeast autolysate agar (CYA), Blakeslee’s malt extract agar (MEA; Bacto), yeast extract sucrose agar (YES), 25 % glycerol nitrate agar (G25N), and creatine sucrose agar (CREA), incubated at 25 °C for 7 d, with additional CYA plates incubated at 30 and 37 °C. CREA contains bromocresol purple and is used as a rapid method for detecting acid production by colonies, indicated by a colour change in the medium from purple to yellow (Frisvad 1981 (link)). Media preparation, inoculation technique, incubation conditions and microscope preparations were standardised according to the recommended methods published in Visagie et al. (2014b) (link). Colour names and codes used in descriptions are based on Kornerup & Wanscher (1967) . An Olympus SZX12 dissecting microscope and Olympus BX50 light microscope equipped with an Evolution MP digital microscope camera and ImagePro v. 6.0 software were used for microphotography. Photographic plates were prepared in Adobe Photoshop CS6.

Visagie C.M., Seifert K.A., Houbraken J., Samson R.A, & Jacobs K. (2016). A phylogenetic revision of Penicillium sect. Exilicaulis, including nine new species from fynbos in South Africa. IMA Fungus, 7(1), 75-117.

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Systematic Study of Insect Morphology

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Morphological terminology used in this work follows Dietrich (2005) (link). Habitus photographs were taken using a Canon EOS 5D Mark II camera and the Camlift V2.7.0 software. Multiple photographs of each specimen were compressed into final images with Zerene Stacker (64-bit) software. Body length was measured from the apex of vertex to the tip of forewings. Abdomens were removed from specimens and cleared in cold 10% KOH solution overnight. The cleared material was rinsed with water and stored in glycerin. An Olympus SZX12 dissecting microscope was used for specimen study and Olympus BX41 and BX53 stereoscopic microscopes were used alternately for drawing of the dissected male genitalia and wings. The holotype of the new species is deposited at the Queen Sirikit Botanical Garden (QSBG), Chiang Mai, Thailand, and additional specimens examined are deposited at the Illinois Natural History Survey (

INHS

), Prairie Research Institute, University of Illinois at Urbana-Champaign, USA (

UIUC

), and the School of Karst Science (

SKS

), Guizhou Normal University, Guiyang, China.

Song Y, & Li C. (2019). A new unique leafhopper genus of Erythroneurini from Thailand, with the description of one new species (Hemiptera, Cicadellidae, Typhlocybinae). ZooKeys, 829, 23-28.

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Standardized Fungal Morphological Characterization

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Morphological characters were captured using standardized protocols proposed by Visagie et al. (2014b (link)). Colony characters were captured on Czapek yeast autolysate agar (CYA), MEAbl, yeast extract sucrose agar (YES), oatmeal agar (OA) and creatine sucrose agar (CREA). Strains were inoculated in a three-point pattern on these media in 90 mm Petri dishes. Plates were incubated for 7 d at 25 °C in darkness in perforated plastic bags. Colour names and codes used in descriptions are from Kornerup & Wanscher (1967) . Microscopic observations were made using an Olympus SZX12 dissecting microscope and Olympus BX50 compound microscope equipped with Infinity3 and InfinityX cameras driven by Infinity Analyze v. 6.5.1 software (Lumenera Corp., Ottawa, Canada). Colonies were captured with a Sony NEX-5N camera. Plates were prepared in Affinity Photo v. 1.6.6 [Serif (Europe) Ltd, Nottingham, UK]. For aesthetic purposes, micrographs were adjusted using the “inpainting brush tool” without altering areas of scientific significance. Line drawings were prepared in Affinity Photo v. 1.7.1 [Serif (Europe) Ltd, Nottingham, UK] running on an iPad Pro with an Apple Pencil.

Visagie C.M., Yilmaz N., Vanderwolf K., Renaud J.B., Sumarah M.W., Houbraken J., Assebgui R., Seifert K.A, & Malloch D. (2019). Penicillium diversity in Canadian bat caves, including a new species, P. speluncae. Fungal Systematics and Evolution, 5, 1-15.

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Quantitative Analysis of Lung Collagen

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Lung sections were stained with Masson’s trichrome reagent to stain collagen. Lung sections were imaged using an Olympus BX50 Light Microscope with Qlmaging Retiga 2000R digital camera. Total lung collagen was measured in the right middle lobe of the lung after overnight digestion with 10 mg/ml pepsin in 0.5 M acetic acid using the Sircol Assay (Biocolor) as directed by the manufacturer. Hydroxyproline content was assessed in the right superior lung lobe⁴⁷. The amount of collagen in tissue sections was quantified using picrosirius red and imaging under polarized light⁴⁸. Paraffin-embedded whole lung tissues (5 μm sections) were processed and stained in 0.1% Sirius red F3BA (Pfaltz & Bauer, S03695) in saturated picric acid. Monochromatic images were taken using an Olympus SZX12 dissecting microscope, in order to reduce selection bias. Square grids were randomly generated to select 10 parenchymal regions. Grids containing edges, blank spaces, airways, or debris, were disregarded. Images of thus randomly selected regions were acquired using an Olympus BX50 Light Microscope at ×20 magnification with circularly polarized light (Olympus). Overall collagen content was assessed using MetaMorph software⁴⁸. The data are expressed as a ratio of the colored pixels representing collagen fibers to the total area, and reflect the average of 10 images/animal.

Anathy V., Lahue K.G., Chapman D.G., Chia S.B., Casey D.T., Aboushousha R., van der Velden J.L., Elko E., Hoffman S.M., McMillan D.H., Jones J.T., Nolin J.D., Abdalla S., Schneider R., Seward D.J., Roberson E.C., Liptak M.D., Cousins M.E., Butnor K.J., Taatjes D.J., Budd R.C., Irvin C.G., Ho Y.S., Hakem R., Brown K.K., Matsui R., Bachschmid M.M., Gomez J.L., Kaminski N., van der Vliet A, & Janssen-Heininger Y.M. (2018). Reducing protein oxidation reverses lung fibrosis. Nature medicine, 24(8), 1128-1135.

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GUS Staining of Seedlings

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SAUR22::GUS and PP2C.D1::EGFP-GUS seedlings at indicated times were used for β-glucuronidase (GUS) staining. Seedlings were incubated in the staining buffer containing 0.1 M sodium phosphate buffer (pH 7), 1 mM K₄Fe(CN)₆, 1 mM K₃Fe(CN)₆, 0.1% Triton X-100, and X-Gluc (1 mg/ml) for 2 hours at 37°C. GUS expression patterns were imaged with an Olympus SZX12 dissecting microscope using SPOT Advanced imaging software.

Du M., Bou Daher F., Liu Y., Steward A., Tillmann M., Zhang X., Wong J.H., Ren H., Cohen J.D., Li C, & Gray W.M. (2022). Biphasic control of cell expansion by auxin coordinates etiolated seedling development. Science Advances, 8(2), eabj1570.

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Fungal Colony Characterization Protocol

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Colony characters were recorded from various media incubated for 7 d, including Czapek yeast autolysate agar (CYA), Blakeslee’s (1915) malt extract agar (MEAbl), yeast extract sucrose agar (YES), oatmeal agar (OA) and creatine sucrose agar (CREA). Bacto^TM malt extract was used for MEAbl preparation. Media preparations, inoculations, incubation conditions and microscopic preparations followed the recommendations by Visagie et al. (2014b) (link). Colour names and codes used in descriptions are from Kornerup & Wanscher (1967) . Microscopic observations were made on an Olympus SZX12 dissecting microscope and Olympus BX50 compound microscope equipped with Infinity3 and InfinityX cameras driven by Infinity Analyze v. 6.5.1 software (Lumenera Corp., Ottawa, Canada).

Visagie C.M., Renaud J.B., Burgess K.M., Malloch D.W., Clark D., Ketch L., Urb M., Louis-Seize G., Assabgui R., Sumarah M.W, & Seifert K.A. (2016). Fifteen new species of Penicillium. Persoonia : Molecular Phylogeny and Evolution of Fungi, 36, 247-280.

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GUS Staining of Plant Tissues

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GUS staining of plant tissues was performed at 37 ^oC in a solution containing 100 mM sodium phosphate (pH 7.0), 10 mM EDTA, 0.5 mM K₄Fe[CN]₆, 0.5 mM K₃Fe[CN]₆, 0.1% triton X-100, and 1 mM X-Gluc (5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide cyclohexylammonium salt, Gold Biotechnology). After removing chlorophyll with 70% ethanol, GUS-stained tissues were cleared in a solution containing 20% lactic acid and 20% glycerol. GUS expression patterns were imaged with an Olympus SZX12 dissecting microscope using the SPOT Advanced imaging software.

Ren H., Park M.Y., Spartz A.K., Wong J.H, & Gray W.M. (2018). A subset of plasma membrane-localized PP2C.D phosphatases negatively regulate SAUR-mediated cell expansion in Arabidopsis. PLoS Genetics, 14(6), e1007455.

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Fossil Specimen Preparation and Analysis

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Fossil specimens were obtained from amber dealers in Palanga, Lithuania. Morphological characters were assessed by examination of the specimens using an Olympus SZX-12 dissecting microscope. Specimens were prepared by grinding flat facets in strategic locations to obtain a clear field of view for detailed photomicrographs according to Nascimbene and Silverstein (2000) and Bisulca et. al. (2012) . Photomicrographs were taken using a Zeiss SteREO Discovery V20 zoom stereomicroscope with a Plan-Apochromat S 0.63x f/Reo WD=81 mm objective. Drawings were prepared either with a camera lucida or by tracing over photographs of the specimens. For bilaterally symmetrical parts of the head and thorax obscured by fractures and other flaws in the amber, drawings (Fig. 2A, B, F, G) were prepared by tracing one half (the fully visible side) with the camera lucida and reconstructing the other half using its mirror image. All specimens examined are deposited in the Paleontological Collection of the Illinois Natural History Survey, Champaign, Illinois, USA. Morphological terminology follows Dietrich (2005) .

Dietrich C.H, & Thomas M.J. (2018). New eurymeline leafhoppers (Hemiptera, Cicadellidae, Eurymelinae) from Eocene Baltic amber with notes on other fossil Cicadellidae. ZooKeys.

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Quail CAM Assay for Angiogenesis

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The chorioallantoic membrane (CAM) assay was performed as described by Chan et al.18 (link). Briefly, fertilized quail (Cortunix cortunix) eggs were purchased from a local supplier (Jimboomba, Queensland, Australia) and incubated at 37 °C for 4 days in a humidified incubator prior to the start of the assay. All peptides were tested at 10 μM, except MCoAA-02, which was tested at concentrations ranging from 0.001 μM to 10 μM with the addition of VEGF (final concentration: 0.3 nM). VEGF solution and DMEM were used as positive and negative controls, respectively. Images were taken on an Olympus SZX12 dissecting microscope with an original magnification of x16. DP capture and DP manager software packages were used during image acquisition. Blood vessel number was quantified by eye, using a hand tally counter. A one-way ANOVA was applied for the statistical comparison analysis using Prism Version 6 software (GraphPad). Each test peptide was compared with the positive control, and results were represented as mean blood vessel count. P < 0.05 was considered significant. All data represents average mean ± SD (n ≥ 6).

Chan L.Y., Craik D.J, & Daly N.L. (2016). Dual-targeting anti-angiogenic cyclic peptides as potential drug leads for cancer therapy. Scientific Reports, 6, 35347.

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