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N a plan fluor apochromat objective

Manufactured by Olympus
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The N.A. Plan Fluor/Apochromat objective is a high-performance microscope objective designed for a wide range of applications. It features a numerical aperture (N.A.) value that optimizes light collection and resolution, making it suitable for various imaging techniques. The objective is constructed using a combination of plan and apochromatic optical elements to provide consistent, distortion-free performance across the field of view.

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Lab products found in correlation

Neurotrace dye, by Thermo Fisher Scientific (2 mentions) Nis elements software, by Nikon (2 mentions)

Close spelling variants of this product

Plan Apochromat objective Plan Fluor objectives Plan Apochromat

2 protocols using n a plan fluor apochromat objective

1

Unbiased Stereological Quantification of Nigral Dopaminergic Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
The total number of surviving dopaminergic neurons in the substantia nigra was determined using an adapted protocol from Tapias et al. (2013), employing an unbiased, automated stereological system. Briefly, 1 in every 6th nigral tissue section were stained for TH and counterstained with NeuroTrace Dye (640; Life Technologies). Tissue sections were scanned and imaged using an Olympus microscope equipped with 20× objective high N.A. plan fluor/apochromat objective. Twenty times magnification was used to generate montage imaging of the ventral midbrain, for which the entire substantia nigra was analyzed per image using anatomical region of interest boundaries. Images were processed using Nikon NIS-Elements software, and quantitative analysis was performed on fluorescent images colocalizing TH and Nissl-positive stains.
Rocha E.M., De Miranda B.R., Castro S., Drolet R., Hatcher N.G., Yao L., Smith S.M., Keeney M.T., Di Maio R., Kofler J., Hastings T.G, & Greenamyre J.T. (2019). LRRK2 inhibition prevents endolysosomal deficits seen in human Parkinson’s disease. Neurobiology of disease, 134, 104626.
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2

Unbiased Stereological Quantification of Nigral Dopaminergic Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
The total number of surviving dopaminergic neurons in the substantia nigra was determined using an adapted protocol from Tapias et al. (2013), employing an unbiased, automated stereological system. Briefly, 1 in every 6th nigral tissue section were stained for TH and counterstained with NeuroTrace Dye (640; Life Technologies). Tissue sections were scanned and imaged using an Olympus microscope equipped with 20× objective high N.A. plan fluor/apochromat objective. Twenty times magnification was used to generate montage imaging of the ventral midbrain, for which the entire substantia nigra was analyzed per image using anatomical region of interest boundaries. Images were processed using Nikon NIS-Elements software, and quantitative analysis was performed on fluorescent images colocalizing TH and Nissl-positive stains.
Rocha E.M., De Miranda B.R., Castro S., Drolet R., Hatcher N.G., Yao L., Smith S.M., Keeney M.T., Di Maio R., Kofler J., Hastings T.G, & Greenamyre J.T. (2019). LRRK2 inhibition prevents endolysosomal deficits seen in human Parkinson’s disease. Neurobiology of disease, 134, 104626.
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