Plasticware such as T-75 culture flasks and Transwell Permeable supports (1.13 cm2, 0.4 μm pore size) were purchased from Corning, Fischer Scientific (Slangerup, Denmark). Hank’s balanced salt solution (HBSS) was purchased from Life Technologies (Taastrup, Denmark), Fetal bovine serum (FBS) was purchased from Gibco, Fischer Scientific (Slangerup, Denmark), penicillin and streptomycin were from Bio Whittaker Cambrex (Vallensbaek, Denmark), while 2-[4-(2-hydroxyethyl) piperazin-1-yl] ethanesulfonic acid (HEPES) was from AppliChem GmbH (Darmstadt, Germany). The radio-labelled isotopes, [3H]-Digoxin (26.3 Ci/mmol) and [14C]-Mannitol (0.057 Ci/mmol), together with Ultima Gold scintillation fluid, were purchased from PerkinElmer (Boston, MA, USA). [3H]-Olanzapine (80 Ci/mmol) and [3H]-Vinblastine (20 Ci/mmol) were from American Radio-Labelled Chemicals (St. Louis, MO, USA). All other compounds and reagents were purchased from Sigma-Aldrich (Broendby, Denmark), unless otherwise stated.
2 4 2 hydroxyethyl piperazin 1 yl ethanesulfonic acid hepes
Manufactured by AppliChem
Sourced in Denmark
2-[4-(2-hydroxyethyl) piperazin-1-yl] ethanesulfonic acid (HEPES) is a zwitterionic organic chemical compound that is commonly used as a buffer solution in biological research and cell culture applications. It maintains a stable pH between 6.8 and 8.2 and is useful for maintaining the physiological pH of cell culture media and other biological systems.
Automatically generated - may contain errors
2 protocols using 2 4 2 hydroxyethyl piperazin 1 yl ethanesulfonic acid hepes
1
Transwell Permeability Assay for Drug Transport
2
In Vitro Permeability Assay
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Fetal bovine serum (FBS) was from Gibco, Fischer Scientific (Slangerup, Denmark), penicillin and Strepotmycin from BioWhittaker Cambrex (Vallensbaek, Denmark), Hank’s balanced salt solution (HBSS) was from Invitrogen (Taastrup, Denmark), and 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES) from AppliChem GmbH (Darmstadt, Germany). Plastic ware such as T-75 culture flasks and Transwell® Permeable supports (1.13 cm2, 0.4 μm pore size) were purchased from Corning, Fischer Scientific.
The radiolabeled isotopes [3H]-digoxin (29.8 Ci/mmol), [14C]-mannitol (0.0571 Ci/mmol), and [3H]-verapamil (0.0815 Ci/mmol) together with Ultima Gold scintillation fluid were purchased from Perkin Elmer Life and Analytical (Boston, MA). Telmisartan was purchased from Sequoia Research Products Ltd. (Pangbourne, UK) Zosuquidar (LY335979, ZSQ) was from Selleck Chemicals (Munich, Germany). All other compounds and reagents were bought from Sigma-Aldrich (Brøndby, Denmark) unless otherwise stated.
The radiolabeled isotopes [3H]-digoxin (29.8 Ci/mmol), [14C]-mannitol (0.0571 Ci/mmol), and [3H]-verapamil (0.0815 Ci/mmol) together with Ultima Gold scintillation fluid were purchased from Perkin Elmer Life and Analytical (Boston, MA). Telmisartan was purchased from Sequoia Research Products Ltd. (Pangbourne, UK) Zosuquidar (LY335979, ZSQ) was from Selleck Chemicals (Munich, Germany). All other compounds and reagents were bought from Sigma-Aldrich (Brøndby, Denmark) unless otherwise stated.
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