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Fitc labeled recombinant human insulin

Manufactured by Merck Group

FITC-labeled recombinant human insulin is a fluorescently-labeled form of the human insulin protein. It is produced through recombinant DNA technology. The FITC (fluorescein isothiocyanate) label allows for the visualization and tracking of the insulin protein in various experimental and research applications.

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2 protocols using fitc labeled recombinant human insulin

1

Peptide Synthesis and Characterization

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FITC-labeled recombinant human insulin (51 a.a.) was purchased from Sigma. Amyloid beta-40 (40 a.a.) was obtained from Peptide Institute (Osaka, Japan). CPPs and C9orf72-Arg-rich dipeptides were synthesized by Genscript (Piscataway, NJ). The purity of the peptides was at least 85%. After synthesis, trifluoroacetate was replaced by acetate.
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2

Insulin Solubilization and Labeling

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Recombinant
human insulin (product number: 91077C) and FITC-labeled recombinant
human insulin (product number: I3661; degree of substitution: 1 mole/mole)
were obtained commercially from Sigma Aldrich. The lyophilized powdered
products were stored in the dark at −20 °C. To dissolve
the insulin, a pH 3 solution was prepared. Buffer solutions of comparable
pH were avoided due to solubility issues, and a rich ionic environment
is known to alter insulin’s molecular conformation.
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