Recombinant human TGF-β1 was purchased from PeproTech (Princeton, NJ, USA). SB431542 was purchased from Selleckchem (Houston, TX, USA). RGD peptide was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). TGF-β neutralizing antibody was purchased from R&D Systems (Minneapolis, MN, USA). Anti-TNC antibodies for IHC staining and western blot analysis were purchased from Santa Cruz Biotechnology and Abcam (Cambridge, UK), respectively. Anti-GAPDH antibody was from AbClone (Seoul, Korea). Anti-SMAD2 (SMAD family member 2), anti-phospho-SMAD2, anti-SMAD3, and anti-phospho-SMAD3 antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-pro-COL1A1 and anti-MMP-1 antibodies have been described previously [49 (link)]. Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG, goat anti-rabbit IgG, and rabbit anti-goat IgG antibodies were purchased from KOMA Biotech (Seoul, Korea). Rhodamine Red-X-conjugated goat anti-mouse IgG and Alexa Fluor 488-conjugated rabbit anti-goat IgG antibodies were purchased from Invitrogen (Carlsbad, CA, USA).
Goat anti rabbit igg
Manufactured by Koma Biotech
Goat anti-rabbit IgG is a secondary antibody used in various immunological techniques. It is produced by immunizing goats with rabbit immunoglobulin G (IgG) and purifying the resulting antibodies. This product can be utilized to detect and quantify rabbit primary antibodies in applications such as Western blotting, ELISA, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase hrp conjugated goat anti mouse igg, by Koma Biotech (3 mentions)
Anti gapdh antibody, by Abclone (3 mentions)
Rhodamine red x conjugated goat anti mouse igg, by Thermo Fisher Scientific (3 mentions)
Anti phosphotyrosine antibody clone 4g10, by Merck Group (2 mentions)
Horseradish peroxidase conjugated goat anti mouse igg, by Koma Biotech (2 mentions)
Tgf β neutralizing antibody, by R&D Systems (2 mentions)
Rgd peptide, by Santa Cruz Biotechnology (2 mentions)
Recombinant human tgf β1, by Thermo Fisher Scientific (2 mentions)
Anti phospho smad3, by Cell Signaling Technology (2 mentions)
Sb431542, by Selleck Chemicals (2 mentions)
Anti smad3, by Cell Signaling Technology (2 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Ab171738, by Abcam (1 mentions)
Anti nf κb p65, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 488 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
Recombinant human tnf α, by Thermo Fisher Scientific (1 mentions)
Anti phospho p38 mitogen activated protein kinase, by Cell Signaling Technology (1 mentions)
Anti p38 mapk, by Santa Cruz Biotechnology (1 mentions)
Anti erk2, by Santa Cruz Biotechnology (1 mentions)
2 7 dichlorofluorescein diacetate dcf da, by Merck Group (1 mentions)
4 protocols using goat anti rabbit igg
1
TGF-β1 Signaling Pathway Regulation
2
Protein Phosphorylation Analysis by Western Blot
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Western blotting was performed as described previously25 (link). The anti-PTK7 antibody was described previously36 (link). Antibodies against phospho-ERK, ERK2, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, Dallas, TX, USA). Antibodies against phospho-Src (Tyr416), Src, phospho-Akt (Ser473), and Akt were purchased from Cell Signaling Technology (Danvers, MA, USA). The anti-phospho-tyrosine antibody (clone 4G10) was purchased from Millipore (Billerica, MA, USA). Horseradish peroxidase-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were purchased from KOMA Biotech (Seoul, Korea).
3
Investigating Inflammatory Signaling Pathways
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Recombinant human TNF-α was purchased from Peprotech and 2′,7′-dichlorofluorescein diacetate (DCF-DA) was purchased from Sigma–Aldrich. The following antibodies were used: anti-SOD3 for western blotting (ab83108) and anti-SOD3 for immunohistochemistry (IHC; ab171738) from Abcam; anti-glyceraldehyde dehydrogenase (GAPDH; ab83108) from AbClone; anti-phospho-NF-κB inhibitor protein ɑ (IκBɑ), anti-phospho-NF-κB p65, anti-phospho-c-jun N-terminal kinase (JNK), anti-JNK, anti-phospho-p38 mitogen-activated protein kinase (MAPK) (Cell Signaling Technology), anti-NF-κB p65, anti-p38 MAPK, anti-phospho-extracellular signal-regulated kinase (ERK), and anti-ERK2 from Santa Cruz Biotechnology; and anti-collagen type I cleavage site from ImmunoGlobe. Anti-MMP-1 and procollagen α1(I) N-propeptide (pN-ColIα1) antibodies have been previously described [21 (link)]. Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were purchased from KOMA Biotech (Seoul, Korea). Alexa Fluor® 488 goat anti-rabbit IgG (H+L) and rhodamine Red-X-conjugated goat anti-mouse IgG were purchased from Thermo Fisher Scientific (Waltham, MA, USA). SPlink HRP Detection Bulk Kit for Mouse and Rabbit Antibodies and Liquid AEC Substrate Kit (20×) for IHC were purchased from GBI labs (Bothell, WA, USA).
4
Regulation of Extracellular Matrix Proteolysis
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Quenched fluorogenic DQ Gelatin and rhodamine-conjugated phalloidin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fluorescein isothiocyanate (FITC)-conjugated gelatin, anti-FLAG antibody, and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Genistein, PP1, PP2, and LY294002 were purchased from AG Scientific (San Diego, CA, USA). TKI-258 was purchased from ApexBio (Houston, TX, USA). PD98059 and SP600125 were purchased from Tocris Bioscience (Bristol, UK). QNZ and antibodies against phospho-ERK, ERK2, c-Fos, lamin A/C, phospho-IKKα (Thr23), IKKα, NF-κB p65, CAS, FAK, Src, and HA tag were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against phospho-JNK, JNK, c-Jun, phospho-Src family (Tyr416), phospho-Akt (Ser473), Akt, phospho-IKKα/β (Ser176/180), IKKβ, phospho-IκBα (Ser32), IκBα, phospho-NF-κB p65 (Ser536), and MMP-9 were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-phosphotyrosine antibody (clone 4G10) and anti–MMP-2 antibody were purchased from Millipore (Billerica, MA, USA). Anti-GAPDH antibody was purchased from AbClone (Seoul, Korea). Horseradish peroxidase-conjugated goat anti-mouse IgG, rabbit IgG, and rabbit anti-goat IgG were purchased from KOMA Biotech (Seoul, Korea). Anti-PTK7 antibody was described previously [39 (link)].
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