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Dulbecco s phosphate buffered saline dpbs 10x

Manufactured by Merck Group
Sourced in United Kingdom

Dulbecco's Phosphate Buffered Saline (DPBS) 10x is a concentrated buffer solution used in various cell culture and biological applications. It maintains the pH and osmotic balance of the sample. The 10x concentration requires dilution before use.

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2 protocols using dulbecco s phosphate buffered saline dpbs 10x

1

Polymeric Nanoparticle-based Chemotherapy

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Ester-terminated 50:50 Poly(DL-lactic-co-glycolidic acid) (PLGA, inherent viscosity range of 0.55–0.75 dL/g) was purchased from Lactel Absorbable Polymers (Durect Corp., Birmingham, AL; catalog number B6010-2P). The PHT-427 chemotherapeutic drug was synthesized following a previously reported protocol.18 (link) Poly(vinyl alcohol) (PVA) that was 87–90% hydrolyzed and with average molecular weight 30,000–70,000 was purchased from Sigma-Aldrich (St. Louis, MO; catalog number P8136). Dichloromethane (DCM) that was 99.8% anhydrous was purchased from Sigma-Aldrich (St. Louis, MO). Paraformaldehyde was purchased from EMD Millipore (Merck KGaA, Darmstadt, Germany). Sesame seed oil was purchased from Sigma-Aldrich (St. Louis, MO). Heparin was purchased from APP Pharmaceuticals, LLC (Shaumburg, IL). Heparinized saline, 50 μl of 3000 U/L, was used for blood samples so they would not coagulate. Acetonitrile that was 99.9% anhydrous was purchased from Acros Organics (Thermo Fisher Scientific, Inc., Waltham, MA). A methyl ester version of tetramethylrhodamine (TMRM) was purchased from Life Technologies (Molecular Probes, Grand Island, NY). Trehalose was purchased from Sigma Aldrich (St. Louis, MO). Sodium Dodecyl Sulfate (SDS) was purchased from OmniPur (EMD Millipore, Darmstadt, Germany). Dulbecco’s Phosphate Buffered Saline (DPBS) 10x was purchased from Sigma Aldrich (St. Louis, MO).
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2

Nitric Oxide Quantification Assay

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Sodium nitrite (NaNO2), ferrous sulfate (FeSO4), sulfuric acid (H2SO4), potassium ferricyanide (K3Fe(CN)6), potassium ferrocyanide (K4Fe(CN)6), a nitrite assay kit (Griess reagent, MAK367), and Dulbecco’s phosphate-buffered saline (DPBS 10X) were received from Sigma Aldrich, Merck. Gold target (diameter: 57 mm, thickness: 0.1 mm) with a purity of 99.99% was purchased from Agar Scientific, Essex, UK. Hank’s balanced salt solution (HBSS 10X), Dulbecco’s modified eagle medium mixture powder (DMEM), antibiotic-antimycotic (Anti-Anti 100X), and fetal bovine serum (FBS) were purchased from Gibco, Thermo Fisher Scientific, Waltham, MA, USA. Diaminofluorescein probe DAF-FM diacetate (D23844) was acquired from Invitrogen, Thermo Fisher Scientific.
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