Itf 2357

9464D or Neuro2A (ATCC, Manassas, VA, USA) Nb cells were grown in Dulbecco's modified Eagle medium (DMEM) with 10% FBS, 1% nonessential amino acids (NEAA), and 55 µm β‐mercaptoethanol. 1E6 cells in 100 µL PBS were inoculated subcutaneously into the mouse flank, and tumor sizes were monitored two to three times weekly using calipers. Tumor volumes were estimated as length × width × height × π/6. Mice were euthanized when tumors reached 2.0 cm in maximal linear dimension. Anti‐murine PD‐1 antibody (Bio‐X‐Cell, Lebanon, NH, USA; #BE0278) was given intraperitoneally (i.p.) twice weekly at 100 µg per dose for 2 weeks, beginning on day 32. Azacytidine (Sigma, St. Louis, MO, USA; 0.5 mg·kg⁻¹ i.p.) and ITF‐2357 (APExBIO, Houston, TX, USA; 2 mg·kg⁻¹ i.p.) were given 5 days per week on alternating weeks, beginning on day 33. Anti‐CD4 antibody (Bio‐X‐Cell, BE0003‐1), anti‐CD8 antibody (Bio‐X‐Cell, BE0223), or their combination was given i.p. twice weekly at 100 µg per dose for each antibody beginning on day 21 and continued thereafter.