Tisseel duo quick

Fibrin matrix was produced by modifying two component fibrin glue (Tisseel® Duo Quick,
Baxter SA, Zurich, Switzerland). Both components of fibrin glue were diluted according
to Bensaid et al^{19 (link)}. To dilute the fibrinogen 1:5 a buffer consisting of 10 ml of de-ionised water
with 73.5 mg of sodium citrate, 16.9 mg sodium chloride, 249.9 mg glycine, 30 000 KIU
aprotinin (Sigma, A-3428, Sigma-Aldrich, Stockholm, Sweden) and 150 mg albumin serum
(Sigma, A-3428, Sigma-Aldrich, Stockholm, Sweden). For dilution of thrombin, a solution
of 10 ml of de-ionised water with 58.8 mg calcium chloride, 87.1 mg sodium chloride,
30.3 mg glycine and 500 mg serum albumin was prepared and diluted 1:5. The two solutions
were then combined as explained below producing a relatively soft fibrin clot^{19 (link)} with 18 mg of fibrinogen per ml and 100 IU of thrombin per ml generating the
optimal solution of 1/100 dilution of thrombin and 1/10 dilution of fibrinogen. Fibrin
matrix was used as a conduit filler and cell transplantation and no adverse effects were observed^{18 (link)}.

Tubular fibrin conduit was molded from two compound fibrin glue (Tisseel® Duo Quick,
Baxter SA, Zurich, Switzerland). Fibrin glue contains; 70-110mg/ml of fibrinogen,
2-9mg/ml of plasma fibronection, 10-50 U/ml of factor XIII, 40-120 µg/ml of plasminogen,
3000 KIU/ml of aprotinin solution, 5 IU/ml of thrombin and 40mmol/l of calcium chloride.
All components were mixed in sterile conditions and a silicone mold with centrally
placed metal rod was used to prepare tubular 14-mm-long conduits with uniform 1-mm-thick
walls and 2 mm lumen as described previously^{18 (link)}. After glue polymerization, the rods and silicone mold were removed and fibrin
glue conduits were stored in sterile Dulbecco’s Minimum Eagle’s Medium at room
temperature. Fibrin conduits prepared in this manner degrade within 4 weeks after transplantation^{18 (link)}.