To detect proteins by immunoblotting, samples run in denaturing or nondenaturing gels were transferred to PVDF membranes (Millipore). The following primary antibodies were used in this study: anti-α4/Pre6 (D. Wolf), anti-Rpt1 (Enzo Life Sciences), anti-Rpt2 (Enzo Life Sciences), anti-Rpt3 (Enzo Life Sciences), anti-Rpt4 (W. Tansey), anti-Rpt5 (Enzo Life Sciences), anti-Rpt6 (C. Mann), anti-Rpn12 (D. Finley), anti-Hsm3, anti-Nas2, and anti-Nas6 (all Hochstrasser lab stocks; Funakoshi et al., 2009 (link)); anti-FLAG (Sigma-Aldrich), anti-GFP/JL8 (TaKaRa), and Pgk1 (Invitrogen). Secondary antibodies used for enhanced chemiluminescence were anti-mouse IgG, horseradish peroxidase (HRP)-linked (from sheep) and anti-Rabbit IgG, HRP-linked (from donkey) (both GE Healthcare).
Anti rpt2
Manufactured by Enzo Life Sciences
Sourced in United States
Anti-Rpt2 is a laboratory reagent that targets the Rpt2 subunit of the 26S proteasome. It is used in research applications to study the function and regulation of the proteasome, a key cellular complex involved in protein degradation.
Automatically generated - may contain errors
Lab products found in correlation
Anti rpt1, by Enzo Life Sciences (2 mentions)
Pvdf membrane, by Merck Group (2 mentions)
Anti rpt5, by Enzo Life Sciences (1 mentions)
Anti gfp jl 8, by Takara Bio (1 mentions)
Anti rpt3, by Enzo Life Sciences (1 mentions)
Anti rpt4, by Enzo Life Sciences (1 mentions)
Anti flag, by Merck Group (1 mentions)
Anti rpn10, by Enzo Life Sciences (1 mentions)
Ecl system, by Thermo Fisher Scientific (1 mentions)
Anti ha 12ca5, by Roche (1 mentions)
Anti ub clone p4d1, by Santa Cruz Biotechnology (1 mentions)
Anti uchl5, by Abcam (1 mentions)
Anti rabbit igg antibody, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase conjugated anti mouse igg antibody, by Thermo Fisher Scientific (1 mentions)
Anti usp14 a300 920a, by Fortis Life Sciences (1 mentions)
2 protocols using anti rpt2
1
Immunoblotting Protein Detection Protocol
2
Immunoblotting of Proteasome Complex
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WCEs from cells were prepared in RIPA buffer and were used for IB. Proteins were separated by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Darmstadt, Germany). The membranes were blocked with 5% nonfat milk and probed with the following antibodies: anti-RPN13 (PW9910; Enzo Life Science), anti-RPT2 (PW5030, Enzo), anti-RPN10 (PW9250, Enzo), anti-RPT1 (PW8315, Enzo), anti-α3 (PW8115, Enzo), anti-α7 (PW8110; Enzo), anti-HA (12CA5, Roche), anti-USP14 (A300-920A, Bethyl Laboratories), anti-UCHL5 (ab124931, Abcam), anti-Ub (clone P4D1, Santa Cruz Biotechnology, Dallas, TX, USA), anti-p53 (R-19, Santa Cruz Biotechnology), anti-cyclin A (clone B-8, Santa Cruz Biotechnology), anti-cyclin B1 (GNS1, Santa Cruz Biotechnology), and an anti-ACTB antibody (A1978, Sigma). The membranes were then incubated with a horseradish peroxidase-conjugated anti-mouse IgG antibody (81-6720, Invitrogen) or anti-rabbit IgG antibody (G21234, Invitrogen) and visualized using the ECL system (Thermo Fisher Scientific).
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