Gold nanostars were characterized using TEM (JEOL JEM-1230) equipped with a Gatan CCD camera and 120 keV accelerating voltage. Samples were prepared by first diluting the nanoparticle solution by 50% using ethanol. This solution was pipetted onto 400 mesh copper grids coated with a thin film of Formvar and carbon (Ted Pella). TEM images were analyzed (Image Pro Analyzer or Image J) to estimate the average overall (ferret) radius before (33.0 ± 8.6 nm, N = 163) and after functionalization (24.8 ± 5.0 nm, N = 149) as well as the tip radius of curvature (before 4.4 ± 0.8 nm, N = 213 and after 4.5 ± 0.5 nm, N = 205 functionalization) where N = the total number of measurements collected, and the average ± standard deviation of these are reported.
Jem 1230
Manufactured by Ametek
Sourced in United Kingdom
The JEM-1230 is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials. It provides a stable and reliable platform for researchers and scientists to study the microstructure and composition of a wide range of samples.
Automatically generated - may contain errors
Lab products found in correlation
Ultrascan 2k x 2k ccd camera, by Ametek (2 mentions)
Formvar and carbon, by Ted Pella (1 mentions)
Field emission scanning electron microscope, by Zeiss (1 mentions)
Ultrascan 4000, by Ametek (1 mentions)
Jem 1200ex, by Ametek (1 mentions)
Ultrascan 2k 2k ccd camera, by Ametek (1 mentions)
Methanolic uranyl acetate, by Electron Microscopy Sciences (1 mentions)
Lead citrate, by Electron Microscopy Sciences (1 mentions)
Jem 2200fs cr, by JEOL (1 mentions)
Orius sc1000, by Ametek (1 mentions)
Vitrobot, by Thermo Fisher Scientific (1 mentions)
Ultrascan 4000 sp, by Ametek (1 mentions)
Nanovan, by Nanoprobes (1 mentions)
Filter paper, by Cytiva (1 mentions)
Tecnai f20, by JEOL (1 mentions)
Uranyl acetate, by Electron Microscopy Sciences (1 mentions)
8 protocols using jem 1230
1
Characterization of Gold Nanostars
2
Transmission Electron Microscopy Imaging
3
Ultrastructural Analysis of Newborn Pig Trachea
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Tracheal segments less than 1 mm3 from newborn pigs were cut, rinsed in cold 1X DPBS, and fixed in sodium cacodylate solution with 2.5% glutaraldehyde. Tissues were post-fixed in 2% osmium tetroxide and en bloc stained with 2.5% uranyl acetate. Next, tissues were dehydrated, infiltrated with Eponate 12, and polymerized at 60 °C for one day. 70 nm sections were cut and counterstained with 5% uranyl acetate and Reynold’s lead citrate. Images were taken using a transmission electron microscope (JEOL, JEM1230) with a Gatan UltraScan 2k x 2k CCD camera.
4
Ultrastructural Analysis of Porcine Trachea
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Tracheal segments (<1 mm3) from newborn pigs were cut, briefly rinsed in cold 1× PBS, and fixed in glutaraldehyde fixation buffer (2.5% glutaraldehyde, 0.1 M sodium cacodylate). Tissues were next postfixed in osmium tetroxide (2%), and en-bloc stained with uranyl acetate (2.5%). Then, tissues were dehydrated through a graded ethanol series, infiltrated with Eponate 12, and polymerized at 60 °C for 24 h. Next, 70-nm-thick sections were cut and counterstained with 5% uranyl acetate and Reynold’s lead citrate. Images were taken using a transmission electron microscope (JEOL, JEM1230) with a Gatan UltraScan 2k × 2k CCD camera.
5
Ultrastructural Analysis of Newborn Pig Trachea
6
Transmission Electron Microscopy of Larvae
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Freshly euthanized larvae were fixed overnight in Karnovsky’s fixative40 , rinsed in 0.1 M sodium cacodylate buffer, post-fixed for one hour in 1% osmium tetroxide, then again rinsed in 0.1 M sodium cacodylate buffer. Larvae were then dehydrated in a graded series of ethanol, infiltrated with and embedded in epoxy resin, and polymerized in a 70 °C oven for 48 hours. To identify areas of interest for TEM analysis, semi-thin sections of 1 µm thickness were cut transversely using an ultramicrotome, stained with toluidine blue and imaged with a Nikon Instruments Eclipse compound light microscope. Ultra-thin sections of 100 nm thickness for TEM analysis were then cut using an ultramicrotome and stained with 33% methanolic uranyl acetate for 15 minutes and lead citrate (Electron Microscopy Sciences, cat # 22410) for 7 minutes. Digital electron micrographs were collected using a JEOL JEM-1230 equipped with a Gatan CCD camera.
7
Nanomaterial Characterization by Negative Staining and Cryo-TEM
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All samples were prepared with in H2O containing 10 % DMSO. Samples for negative staining were applied to glow‐discharged carbon‐coated copper grids and stained with 2 % (w/v) NanoVan (methylamine vanadate), Nanoprobes. Digital micrographs were taken at room temperature in low‐dose radiation mode on a Jeol transmission electron microscope (JEM‐1230) operated at 100 kV and equipped with an Orius SC1000 (4008×2672 pixels) cooled slow‐scan CCD camera (GATAN, UK). For cryo‐microscopy studies the samples were vitrified on Quantifoil 2/2 grids, using a Vitrobot (FEI) and were analyzed at liquid nitrogen temperature with a transmission electron microscope operated at 200 kV in low‐dose conditions. The samples were applied to glow‐discharged carbon‐coated copper grids and stained with 2 % (w/v) NanoVan. Micrographs were taken at a low radiation dose on a JEM‐2200FS/CR transmission electron microscope (JEOL, Japan), equipped with an UltraScan 4000 SP (4008×4008 pixels) cooled slow‐scan CCD camera (GATAN, UK).
8
Negative Staining TEM of Virus Samples
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Samples for transmission electron microscopy (TEM) were prepared by the negative staining method using a 2% w/v solution of uranyl acetate (Electron Microscopy Sciences) in distilled water. Virus samples were diluted to approximately 20–100 μg/mL protein in Tris-buffered saline (10 mM Tris, 150 mM NaCl, pH = 7.5). Briefly, 3 μL of diluted virus sample was applied to glow discharged (PELCO easiGlow, TED PELLA) formvar/copper support film TEM grids (Electron Microscopy Sciences). Following a brief incubation of 30 s, the sample was blotted away from the grid using filter paper (Whatman). Sample-laden grids were washed twice by contact with two successive droplets of distilled water, and subsequently dabbed twice into droplets of uranyl acetate solution. Micrographs were obtained using a Hitachi 7,500 (Hitachi) TEM at the Icahn School of Medicine at Mount Sinai, and a Tecnai F20 (Field Electron and Ion company) and JEOL JEM-1230 (Gatan) TEMs at the New York Structural Biology Center.
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