Glucose Uptake Colorimetric Assay (Cat# K676, BioVision), ATP Colorimetric Assay (Cat# K354, BioVision) and Lactate Assay II (Cat# K627, Bio, BioVision) were used to measure the glucose uptake, ATP and lactate production. Glucose uptake colorimetric analysis was carried out in 96‐well plates with a density of 1 × 104 cells per well. 2‐Deoxy‐d‐glucose (2‐DG) (2.5 mmol/L) and sorafenib (6 μmol/L) were added to the plate for 6 hours. Pre–culture of the cells with 100 μL Krebs‐Ringer‐Phosphate‐HEPES buffer containing 2% BSA for 40 minutes resulted in glucose deficiency. The supernatant was obtained by centrifugation to remove the insoluble fraction and ready for assay.
Glucose uptake colorimetric assay
Manufactured by Abcam
The Glucose Uptake Colorimetric Assay is a quantitative biochemical test that measures the uptake of glucose by cells. The assay is based on the detection of a colored product formed from the reduction of a tetrazolium dye, which is proportional to the amount of glucose transported into the cells.
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Lab products found in correlation
3 protocols using glucose uptake colorimetric assay
1
Colorimetric Assays for Metabolic Analysis
2
Metabolic Profiling of Cancer Cells
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The glucose uptake colorimetric assay, lactate colorimetric assay and ATP colorimetric assay kits (Biovision, Inc.) were used to examine glucose consumption and lactate and ATP production in A549 and H1299 cells, according to the manufacturer's protocols.
3
Glucose and Lactate Assay for Cancer Cells
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The glucose uptake colorimetric assay (Biovision) and the lactate colorimetric assay (Biovision) kits were used to detect glucose consumption and lactate production in GC cells, according to the manufacturer’s instructions.
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