Mouse anti β actin antibody

HST, hesperidin (HSD), rutin (RUT), QUE, AICAR (5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside) and LY294002 were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). NAR and NRG were purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). NRT and neohesperidin (NHD) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). All compounds used for NMR and cell experiments were dissolved into d₆-dimethylsulfoxide (DMSO) as a 10 mM solution and stored at −20 °C until needed.
The rabbit anti-CLD-2 antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA). The rabbit anti-ZO-1 antibody was obtained from Invitrogen (Carlsbad, CA, USA). Mouse anti-β-actin antibody was purchased from Wako. For immunofluorescent microscopy, the anti-rabbit immunoglobulin G (IgG) and the F (ab’) 2 fragment-Cy3 antibody were obtained from Sigma-Aldrich. For Western blotting analysis, anti-rabbit and mouse IgG horseradish peroxidase (HRP) conjugates were acquired from Promega (Madison, WI, USA).

HST (>96% pure), QUE (practical grade), SB431542, and U0126 were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). All compounds used for NMR and cell experiments were dissolved into d₆-dimethylsulfoxide (DMSO) as a 100 mM solution and stored at −20 °C until needed.
The rabbit anti-CLD-2 antibody and anti-OCLN antibody were obtained from Sigma-Aldrich (St. Louis, MO, USA). The rabbit anti-ZO-1 antibody was obtained from Invitrogen (Carlsbad, CA, USA). Mouse anti-β-actin antibody was purchased from Wako. Rhodamine–phalloidin was purchased from Cytoskeleton, Inc. (Denver, CO, USA). For immunofluorescence microscopy, the anti-rabbit immunoglobulin G (IgG) and the F(ab’)2 fragment-Cy3 antibody were obtained from Sigma-Aldrich. For Western blotting analysis, anti-rabbit and mouse IgG horseradish peroxidase (HRP) conjugates were acquired from Promega (Madison, WI, USA).