Total RNA was isolated from Gingival MSCs using Trizol reagents (Invitrogen, USA) and reverse transcribed into cDNA using PrimeScript™RT reagent Kit with gDNA Eraser (Takara, Dalian, China). The real-time PCR reactions were performed using the SYBR Premix Ex Taq™ II (Takara, Dalian, China) and IcycleriQ Multi-color Real-time PCR Detection System. The primers were synthesized by Sangon Biotech: Sp1 (5′-TGAGACAGCAGGTGGAGAAG-3′, 5′-GGCTCTTCCCTCACTGTCTT-3′); tert (5′-TGCTGGACACTCAGACTTTGGA-3′, 5′-TTCAACCGCAAGACCGACA-3′); GAPDH (5′-TGAAGCAGGCATCTGAGGG-3′, 5′-CGAAGGTGGAAGAGTGGGAG-3′). Primers for miR-21 and U6 are purchased from RiboBio (Guangzhou, China).
Icycleriq multi color real time pcr detection system
Manufactured by Takara Bio
Sourced in China
The IcycleriQ Multi-color Real-time PCR Detection System is a laboratory instrument designed for real-time polymerase chain reaction (PCR) analysis. It can detect and quantify multiple targets simultaneously using different fluorescent dyes.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Sybr premix ex taq 2, by Takara Bio (1 mentions)
Primescript rt reagent kit with gdna eraser, by Takara Bio (1 mentions)
Primers for mir 21 and u6, by RiboBio (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
Sybr premix ex taqtm, by Takara Bio (1 mentions)
Gdna eraser, by Takara Bio (1 mentions)
2 protocols using icycleriq multi color real time pcr detection system
1
Quantifying Gingival MSC Gene Expression
2
Quantitative Analysis of Gene Expression in hPDLSCs
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Trizol reagent (Invitrogen, USA) was used to extract the total RNA from hPDLSCs. Then, the total DNA was reversely transcribed into cDNA with PrimeScript™ RT Reagent Kit and gDNA Eraser (Takara, Dalian, China). Later, SYBR Premix Ex TaqTM (Takara, Dalian, China) and iCycler iQ Multicolor Real-Time PCR Detection System were used to perform the real-time PCR reactions. The primers synthesized by Sangon Biotech are shown in Table 1 .
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