Cp sil 8cb column

The fatty acid profile of the olive oil samples was determined using a Chrompack CP 9002 system equipped with Agilent Cp-Sil 8 CB column (60 m length, 0.25 mm i.d) (5% phenyl+ 95% dimethyl polysiloxane). The fatty acid methyl esters (FAMEs) were prepared by cold transesterification method according to the chromatographic procedure defined by EEC Regulation 2568/91 [21 ]. Then 0.8 μl was injected under a constant injector temperature of 250°C. The oven temperature increased from 150C° to 200°C by 4°C/min. The nitrogen was used as carrier gas with flow rates of 1ml/min. FAMEs were detected with a flame ionization detector (FID) set at 250°C, and the identification was carried out by comparing their retention times with those of standard reference compounds.

Cellulose pyrolyzed under nitrogen was hydrolyzed by acid hydrolysis. Aqueous H₂SO₄ (72 wt%, 0.1 mL) was added to the residue and the mixture was then heated at 30 °C for 60 min with frequent agitation with a glass rod. The mixture was diluted with water (2.8 mL) and then heated in an autoclave at 121 °C for 60 min. After washing with distilled water and drying, the hydrolyzed residue (solid hydrolyzed products) was analyzed using a portable Curie-point injector (JCI-22, Japan Analytical Industry, Tokyo, Japan) coupled to a Shimadzu-2010 Plus gas chromatograph (Shimadzu Corporation, Kyoto, Japan) and a Shimadzu QP 2010 Ultra mass spectrometer (Shimadzu Corporation, Kyoto, Japan). The hydrolyzed residue was pyrolyzed at 723 °C for 5 s. The instrument conditions were as follows: Agilent CPSil 8CB column (length: 30 m, diameter: 0.25 mm); 250 °C injector temperature; 1 : 50 split ratio; helium carrier gas (1.0 mL min⁻¹). The column temperature was initially set at 50 °C for 3 min, after which it was ramped at 6 °C min⁻¹ to 200 °C and 30 °C min⁻¹ to 300 °C, before being held constant at 300 °C for 5 min. The MS scan parameters included a scan range of 35–600 m/z and a scan interval of 0.3 s.