Anti il 10 apc clone jes3 19f1

Manufactured by BD

Sourced in United States

Anti-IL-10-APC (clone JES3-19F1) is a monoclonal antibody that binds to interleukin-10 (IL-10) and is conjugated to the fluorescent dye allophycocyanin (APC). It is used as a tool in flow cytometry and other immunoassays for the detection and quantification of IL-10.

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Lab products found in correlation

Poly l lysine coated coverslips, by BD (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Tcs sp8 confocal laser scanning microscope, by Leica (1 mentions) Hla dr fitc clone l243, by BD (1 mentions)

Spelling variants of this product

IL-10 (156 citations) Anti-IL-10 (27 citations) IL-10-APC (17 citations) Anti-IL-10-APC (9 citations) IL-10 (clone JES3-19F1) (4 citations)

2 protocols using anti il 10 apc clone jes3 19f1

Multiparameter Flow Cytometry Analysis

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Cells were cultured and restimulated with PMA, ionomycin and BFA for 4 h on day of experiment and stained with fluorescently labeled mAbs against HLA-DR-FITC (clone L243, BD Biosciences). Cells were then fixed in 4% PFA, permeabilized using 0.5% saponin and stained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), anti-TNF-DyLight549 (labeled in-house) and anti-IL-10-APC (clone JES3-19F1, BD Biosciences) antibody for 30 min in the dark. After staining, cells were left to attach to poly-L-Lysine-coated coverslips (BD Biosciences) for 90 min at 20°C. Coverslips were mounted on a microscope slide using VectaShield (Vector Labs), sealed with clear nail polish (Rimmel, London) and analyzed using a Leica TCS SP8 Confocal Laser Scanning Microscope (Leica). Acquired images were analyzed using Fiji software (build #3476, open source).

Lighaam L.C., Unger P.P., Vredevoogd D.W., Verhoeven D., Vermeulen E., Turksma A.W., ten Brinke A., Rispens T, & van Ham S.M. (2018). In vitro-Induced Human IL-10+ B Cells Do Not Show a Subset-Defining Marker Signature and Plastically Co-express IL-10 With Pro-Inflammatory Cytokines. Frontiers in Immunology, 9, 1913.

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Multiparametric Immune Phenotyping

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The antibodies used for staining were immunoglobulin FITC, PE, and PERCP controls (BD, San Diego, CA, USA), anti-CD4 (APC-Cy7- Clone RPA-T4), anti-CD25 (PE-Cy7-Clone M-A251), anti-CD39 (PE-Clone TU66), anti-CD73 (APC- Clone- AD2), and anti-PD1 (FITC- Clone MIH4) obtained from BD Biosciences (USA), along with anti-FOXP3 (Alexa fluor 488- Clone 236A/E7, Alexa Fluor 647- Clone 236A/E7), anti-Perforin (PE- Clone Delta G9), anti-Granzyme B (FITC- Clone GB11), anti-CTLA-4 (CD152) (PE- Clone BNI3), anti-TGF-β (PE- Clone TW4–2F8), anti-IL10 (APC- Clone JES3–19F1) and anti-IFN-γ (PE- Clone B27) antibodies (BD Biosciences, Thermo Fisher Scientific, USA).

Guerra-Gomes I.C., Gois B.M., Peixoto R.F., de Sousa Palmeira P.H., de Sousa Dias C.N., Csordas B.G., Galvão Araújo J.M., Veras R.C., de Medeiros I.A., Assunção Araújo de Azevedo F.D., Boyton R.J., Altmann D.M, & Keesen T.S. (2021). Phenotypical characterization of regulatory T cells in acute Zika infection. Cytokine, 146, 155651.

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