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Annexin 5 propidium iodide dual staining kit

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The Annexin V/Propidium iodide (PI) dual staining kit is a laboratory tool used to detect and quantify cell apoptosis and necrosis. It contains Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a DNA-binding dye. This combination allows for the simultaneous identification of early apoptotic, late apoptotic, and necrotic cells through flow cytometry analysis.

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Lab products found in correlation

Facscan flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Annexin-V fluorescein isothiocyanate (FITC)/propidium iodide (PI) dual-staining kit Annexin V/propidium iodide (PI) staining Annexin V/propidium iodide (PI) Propidium iodide (PI) staining Annexin V/propidium Annexin V staining Propidium iodide Annexin V kit Annexin V

2 protocols using annexin 5 propidium iodide dual staining kit

1

Quantifying Cell Apoptosis by Flow Cytometry

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The percentages of cell apoptosis were quantified by Annexin V/Propidium iodide (PI) dual staining kit (BD Biosciences, San Diego, CA). Samples were analyzed by a FACScan flow cytometer (BD Biosciences). Data were analyzed using the FlowJo software version 10 (FloJo Data Analysis Software, Ashland, Ore).
Chen M., Zhang F., Song J., Weng Q., Li P., Li Q., Qian K., Ji H., Pietrini S., Ji J, & Yang X. (2021). Image-Guided Peri-Tumoral Radiofrequency Hyperthermia-Enhanced Direct Chemo-Destruction of Hepatic Tumor Margins. Frontiers in Oncology, 11, 593996.
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2

Quantifying Cell Apoptosis via Annexin V/PI

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Cell apoptosis was quantified by staining the cells with annexin V/propidium iodide (PI) Dual Staining Kit (BD Biosciences; San Diego, CA). Twenty-four hours after treatments both adherent and floating cells in the four groups were incubated with a mixture of annexin V reagent and propidium iodide. Samples were analyzed by a FACScan flow cytometer (BD Biosciences; San Jose, CA). Data were analyzed using the FlowJo software version 10 (FloJo Data Analysis Software; Ashland, OR).
Qian K., Chen M., Zhang F., Chick J.F., Ji H., Zheng C, & Yang X. (2021). Image-Guided Radiofrequency Hyperthermia (RFH)-Enhanced Direct Chemotherapy of Hepatic Tumors: The Underlying Biomolecular Mechanisms. Frontiers in Oncology, 10, 610543.
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