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Mesencult osteogenic differentiation medium

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MesenCult Osteogenic Differentiation medium is a cell culture medium designed to support the osteogenic (bone) differentiation of mesenchymal stem cells (MSCs) in vitro. The medium provides the necessary components and growth factors to promote the differentiation of MSCs into the osteogenic lineage.

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Lab products found in correlation

Mesencult adipogenic differentiation kit, by STEMCELL (3 mentions) Mesencult acf chondrogenic differentiation medium, by STEMCELL (3 mentions) Mesencult acf basal medium, by STEMCELL (3 mentions) Acf enzymatic dissociation inhibition solution, by STEMCELL (2 mentions)

3 protocols using mesencult osteogenic differentiation medium

1

Multilineage Differentiation of MSCs

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Example 13

Adipocytes, osteocytes, and chondrocytes were generated from MSCs. For adipogenic lineage differentiation, MSCs between passages 3-4 were plated on 6-well plates with MesenCult-ACF Basal Medium (Stem Cell Technologies, Cat. #05449) at 4-5×105 cells per well. Differentiation was performed using the MesenCult Adipogenic Differentiation Kit (Stem Cell Technologies, Cat. #05412) according to manufacturer's protocol. Media changes were conducted every 3-4 days until day 13. For osteogenic lineage differentiation, MSCs between passages 3-4 were plated on a 6-well plate with MesenCult-ACF Basal Medium (Stem Cell Technologies) at 3-4×104 cells per well. Differentiation was performed using the MesenCult Osteogenic Differentiation medium (Stem Cell Technologies, Cat. #05465) according to manufacturer's protocol. Medium changes were conducted every 3-4 days until day 13. For 3D pellet chondrogenic differentiation, MSCs were first released from T25 flasks using ACF Enzymatic Dissociation/Inhibition Solutions (Stem Cell Technologies, Cat. #05426) and collected in polypropylene tubes at 2.5-3×106 cells per tube with MesenCult-ACF Chondrogenic Differentiation Medium (Stem Cell Technologies, Cat. #05455) according to manufacturer's protocol. Medium changes were conducted every 3-4 days until day 13.

US11639510B2. Devices and methods for mitochondria replacement and for generating cellular therapeutics (2023-05-02). NanoCav, LLC [US]. Inventors: Ting-Hsiang Sherry Wu [US], Artin Mehrabi [US], Jon Thomas Van Lew [US].
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2

Multilineage Differentiation of Mesenchymal Stem Cells

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Adipocytes, osteoblasts, and chondrocytes were generated from MSCs. For adipocyte differentiation, MSCs between passages 3 – 4 were plated on 6-well plates with MesenCult-ACF Basal Medium (StemCell Technologies, Cat. # 05449) at 4 – 5 3 105 cells per well. Differentiation was performed using the MesenCult Adipogenic Differentiation Kit (StemCell Technologies, Cat. # 05412) according to the manufacturer’s protocol. Media changes were done every 3 – 4 d until 13 d. For osteogenic lineage differentiation, MSCs between passages 3 – 4 were plated on a 6-well plate with MesenCult-ACF Basal Medium (StemCell Technologies) at 3 – 4 × 104 cells per well. Differentiation was performed using MesenCult Osteogenic Differentiation medium (StemCell Technologies, Cat. # 05465) according to the manufacturer’s protocol. Medium changes were done every 3 – 4 d until 13 d. For 3-D pellet chondrogenic differentiation, MSCs were first released from T25 flasks using ACF Enzymatic Dissociation/Inhibition Solution (StemCell Technologies, Cat. # 05426) and collected in polypropylene tubes at 2.5 – 3 × 106 cells per tube with MesenCult-ACF Chondrogenic Differentiation Medium (StemCell Technologies, Cat. # 05455) according to the manufacturer’s protocol. Medium changes were done every 3 – 4 days until 13 d.
Patananan A.N., Sercel A.J., Wu T.H., Ahsan F.M., Torres A J.r., Kennedy S.A., Vandiver A., Collier A.J., Mehrabi A., Van Lew J., Zakin L., Rodriguez N., Sixto M., Tadros W., Lazar A., Sieling P.A., Nguyen T.L., Dawson E.R., Braas D., Golovato J., Cisneros L., Vaske C., Plath K., Rabizadeh S., Niazi K.R., Chiou P.Y, & Teitell M.A. (2020). Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates. Cell reports, 33(13), 108562.
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3

Multilineage Differentiation of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adipocytes, osteoblasts, and chondrocytes were generated from MSCs. For adipocyte differentiation, MSCs between passages 3 – 4 were plated on 6-well plates with MesenCult-ACF Basal Medium (StemCell Technologies, Cat. # 05449) at 4 – 5 3 105 cells per well. Differentiation was performed using the MesenCult Adipogenic Differentiation Kit (StemCell Technologies, Cat. # 05412) according to the manufacturer’s protocol. Media changes were done every 3 – 4 d until 13 d. For osteogenic lineage differentiation, MSCs between passages 3 – 4 were plated on a 6-well plate with MesenCult-ACF Basal Medium (StemCell Technologies) at 3 – 4 × 104 cells per well. Differentiation was performed using MesenCult Osteogenic Differentiation medium (StemCell Technologies, Cat. # 05465) according to the manufacturer’s protocol. Medium changes were done every 3 – 4 d until 13 d. For 3-D pellet chondrogenic differentiation, MSCs were first released from T25 flasks using ACF Enzymatic Dissociation/Inhibition Solution (StemCell Technologies, Cat. # 05426) and collected in polypropylene tubes at 2.5 – 3 × 106 cells per tube with MesenCult-ACF Chondrogenic Differentiation Medium (StemCell Technologies, Cat. # 05455) according to the manufacturer’s protocol. Medium changes were done every 3 – 4 days until 13 d.
Patananan A.N., Sercel A.J., Wu T.H., Ahsan F.M., Torres A J.r., Kennedy S.A., Vandiver A., Collier A.J., Mehrabi A., Van Lew J., Zakin L., Rodriguez N., Sixto M., Tadros W., Lazar A., Sieling P.A., Nguyen T.L., Dawson E.R., Braas D., Golovato J., Cisneros L., Vaske C., Plath K., Rabizadeh S., Niazi K.R., Chiou P.Y, & Teitell M.A. (2020). Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates. Cell reports, 33(13), 108562.
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