ADAMTS4 release in the hNPCs was determined by enzyme-linked immunosorbent assay (ELISA) using a commercially available ELISA set (R&D Systems Inc., Minneapolis, MN and BD Biosciences, San Diego, CA). ELISA was performed according to the manufacturer's instructions. All samples and standards were measured in duplicate.
Elisa set
Manufactured by R&D Systems
Sourced in United States
The ELISA set is a laboratory equipment designed for the detection and quantification of specific analytes in a sample using the Enzyme-Linked Immunosorbent Assay (ELISA) technique. The set contains the necessary components, such as microplates, antibodies, and reagents, to perform ELISA assays.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using elisa set
1
ADAMTS4 Release in hNPCs
2
ELISA Determination of Inflammatory Cytokines
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TNF-α, IL-1β, IL-6, and IL-8 were determined by enzyme-linked immunosorbent assay (ELISA) using a commercially available ELISA set (R&D Systems Inc., Minneapolis, MN, USA). ELISA was performed according to the manufacturer’s instructions. All samples and standards were measured in duplicate.
3
Magnolol Modulates Inflammatory Cytokines
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Magnolol (with purity ≥95%) was bought from Sigma-Aldrich (St. Louis, MO, USA). Magnolol was dissolved in 40% (v/v) propylene glycol and then dissolved in normal saline to the desired concentration. The final concentration of propylene glycol in the administered Magnolol solution was 4 × 10 -3 % (v/v). The serum levels of TNF-α, IL-1β, IL-6, and IL-10 were determined by using a commercially available ELISA set (R&D Systems, Minneapolis, MN, USA). The diaminobenzidine chromogen used for the terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling (TUNEL) staining was purchased from Boehringer (Mannheim, Germany).
4
Quantitative TGF-β1 and TNF-α Assay
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Quantitative TGF-β1 level determination in the spleen and TNF-α in the hippocampus were evaluated using a standard ELISA according to the manufacturer’s instructions (ELISA Set, R&D Systems). Values were expressed in pg/ml. Optical density was measured at 450 nm within 10 min and protein concentrations were determined using a provided standard curve.
5
CXCL1 and Cytokine Profiling of Tumor Cells
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Tumor cells supernatants were collected as mentioned before. Human CXCL1 expression was analyzed using Elisa set (R & D system, DY 275), according to company instruction. The culture supernatants were assayed at a dilution within the linear range of the CXCL1 standards, and the concentration of CXCL1 in each sample was determined using a standard curve, as indicated by the kit.
Multiplex cytokines analysis was performed using tumor cell supernatants by human 71-cytokine /chemokine array panel kit (Milipore corporation, Burlington, MA, USA). Experimental and analytical steps were carried out at Eve technology corporation (Calgary, AB, Canada).
Multiplex cytokines analysis was performed using tumor cell supernatants by human 71-cytokine /chemokine array panel kit (Milipore corporation, Burlington, MA, USA). Experimental and analytical steps were carried out at Eve technology corporation (Calgary, AB, Canada).
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