RAW264.7 cells were purchased from Cell Resource Center, Peking Union Medical College (Beijing, China). Human adipose-derived stem cells (hADSCs) were donated from School of Basic Medicine Peking Union Medical College. RANKL (462-TEC-010) was were supplied by R&D Systems (Minneapolis, MN, USA). β-glycerophosphate disodium salt hydrate (G9422), l -ascorbic acid (A4544), dexamethasone (D4902), 3-isobutyl-1-methylxanthine (I5879) were bought from Sigma Aldrich Inc. (Merck KGaA, Germany). PF was bought from Nanjing Dilger Medical Technology (CAS: 23180-57-6; purity >98 %). Enzyme-linked immunosorbent assay (ELISA) kits for P1NP (QS47784), CTX-1 (QS47767), BALP (QS440290), and TRAP (QS440351) were obtained from Beijing gersion Bio-Technology Co., Ltd. HDL-C (A112-1-1) and LDL-C (A113-1-1) kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). NFATc1 (66963-1-Ig), cathepsin K (11239-1-AP), C/EBPα (18311-1-AP), and PPARγ (16643-1-AP) antibodies were purchased from Proteintech Group (Hubei China). MEDAG (orb326838) antibody was purchased from biorbyt (UK). Phospho (p)-AMPKα (Thr172) (2535S) antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). Runx2 (ab236639) and OPG (ab73400) antibodies were purchased from Abcam (Cambridge, UK).
Rankl 462 tec 010
Manufactured by R&D Systems
Sourced in United States
RANKL (462-TEC-010) is a recombinant human protein that belongs to the tumor necrosis factor (TNF) ligand superfamily. It is a key regulator of osteoclast differentiation and activation.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Pparγ 16643 1 ap, by Proteintech (1 mentions)
Quercetin que, by Merck Group (1 mentions)
Curcumin cur, by Merck Group (1 mentions)
Iscript cdna synthesis kit for rt qpcr, by Bio-Rad (1 mentions)
M csf 416 ml 010, by R&D Systems (1 mentions)
Rnazol, by Molecular Research Center (1 mentions)
Realtime glo mt cell viability assay, by Promega (1 mentions)
Tert butylhydroquinone tbhq, by Merck Group (1 mentions)
2 protocols using rankl 462 tec 010
1
In vitro Osteogenic Differentiation Assay
2
Osteoclastogenesis Induction Protocol
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Receptor activator of nuclear factor kappa-B (NF-κB) ligand (RANKL, #462-TEC-010) and macrophage colony-stimulating factor (M-CSF, #416-ML-010) were obtained from R&D Systems (Minneapolis, MN). The RealTime-Glo™ MT Cell Viability Assay was purchased from Promega (Madison, WI). RNAzol was purchased from the Molecular Research Center (Cincinnati, OH). The iScript™ cDNA Synthesis Kit for RT-qPCR was purchased from Bio-Rad (Hercules, CA). The dye 2′,7′-dichlorofluorescin diacetate (DCF, #D6883) for ROS measurement was purchased from Sigma-Aldrich (St. Louis, MO). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin, and streptomycin were all obtained from Thermo Fisher Scientific (Waltham, MA). The NRF2 inducers curcumin (CUR, #C1386), quercetin (QUE, #Q4951), bardoxolone methyl (CDDO-Me, #SMB00376), sulforaphane (SFN, #S4441), and tert-butylhydroquinone (tBHQ, #112941) were all purchased from Sigma-Aldrich.
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