Chloroform

BMAA hydrochloride, L-2,4-diaminobutyric acid (DAB) dihydrochloride, trichloroacetic acid (TCA), 5-dimethylamino-1-naphthalenesulfonyl chloride/dansyl chloride (DNS), formic acid, acetonitrile, methanol, hydrochloric acid (HCl) [37%], chloroform and Corning^® Costar^® Spin-X^© centrifuge filters were purchased from Sigma-Aldrich Company Ltd (Gillingham, United Kingdom). All chemicals used were of analytical grade or higher. The water was purified using a Milli-Q purification system from Merck Millipore (Merck KGaA, Darmstadt, Germany). Deuterium labelled BMAA (D₃-BMAA) was a gift from Johan Rosén at The National Food Agency in Sweden, and was synthesized as described in the publication by Rosén and Hellenäs12 (link). Stock solutions of BMAA (1 mg/ml) were prepared in Milli-Q water and stored at −20˚C.

Poly(d,l-lactide-co-glycolide) (PLGA; 50:50, inherent viscosity of 0.95–1.20
dL/g), poly(d,l-lactide-co-glycolide)
(PLGA; 75:25, inherent viscosity of 0.80–1.20 dL/g), and poly(l-lactide) (PLA, inherent viscosity of 0.90–1.20 dL/g)
were purchased from Evonik (Birmingham, AL). Potassium permanganate,
chloroform, 30% hydrogen peroxide (H₂O₂), agarose
(Low-EEO/Multi-Purpose/Molecular Biology grade), Corning RPMI 1640
Medium (Mod.) 1X with l-glutamine, Gibco Trypsin-EDTA (0.25%),
Promega CellTiter 96TM AQueous One Solution Cell Proliferation Assay
(MTS), poly(vinyl alcohol) (PVA; 80% hydrolyzed, MW = 9,000–10,000),
sucrose, and paraformaldehyde were purchased from Sigma-Aldrich (St.
Louis, MO). LIVE/DEAD viability/Cytotoxicity Kit for mammalian cells,
Alexa Fluor 594 NHS Ester (Succinimidyl Ester), and Image-iT Green
Hypoxia Reagent were purchased from Thermofisher (Waltham, MA). HO-PEG-5000-NHS
was purchased from Sigma-Aldrich. All cell lines used in this study
were purchased from ATCC (Manassas, VA). GeneJet RNA isolation kit
for mRNA extraction was obtained from VWR (Radnor, PA). SuperScript
III First-Strand Synthesis System used for cDNA synthesis was purchased
from Thermofisher (Waltham, MA). AriaMx Real-time PCR System and Ultra-Fast
SYBR Green Low ROX QPCR Master Mix were purchased from VWR (Radnor,
PA).