Circular dichroism (CD) experiments were carried out at a constant temperature of 25 °C with a Jasco J-810 spectropolarimeter equipped with a Model PTC-423S/L Peltier type temperature controller. A 0.1 cm path-length cuvette was used. Each sample was scanned at least three times to obtain the average within the wavelength range of 190–250 nm, and points were taken in 1 nm intervals. The enzyme solution was prepared at a concentration of 1.0 mg/mL in 20 mM potassium phosphate buffer (pH = 7). TZQ concentrations were between 0.07 and 0.28 g/L, and acarbose was used at 0.5 g/L. CD spectra were measured at different time intervals after the addition of TZQ or acarbose into the enzyme solution. The secondary structures of maltase, including α-helices, β-sheets, β-turns and random coils, were analysed by the Chen-Yang program.
Ptc 423s l
Manufactured by Jasco
Sourced in Japan
The PTC-423S/L is a laboratory instrument designed for temperature control and monitoring. It provides precise temperature regulation and measurement capabilities for various applications in scientific research and analysis.
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Lab products found in correlation
2 protocols using ptc 423s l
1
Circular Dichroism Analysis of Maltase
2
Circular Dichroism Spectroscopy of Protein Thermal Stability
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CD spectra were recorded on a Jasco J-715 spectropolarimeter equipped with Peltier-type temperature control system model PTC-423S/L ("Jasco", Japan). The cells had a light path of 0.02 and 1.0 cm, and protein concentration was 0.25 (far-UV CD) and 1.27 mg/mL (near-UV CD). CD spectra were acquired in 50 mmol/L cacodylate, 1 mmol/L CaCl 2 , pH 7.5 buffer. For continuous melting of the samples, the temperature was increased at a rate of 1 K/min (as for DSC), and the transition temperatures were determined from the peaks on the first temperature derivatives of the melting profiles. The results were expressed as molar ellipticity, [⌰] (deg cm 2 /dmol), on the basis of a mean amino acid residue weight (MRW) of 108 (Garnier et al. 1998 ). The molar ellipticity was determined as [⌰] = ( × 100 MRW)/(cl), where c is the protein concentration in milligrams per millilitre, l is the light path length in centimeters, and is the measured ellipticity in degrees.
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