Mir 203 mimic

BPI-9016M, which is a selective small-molecular c-Met inhibitor, was kindly provided by Better Pharmaceuticals (Zhejiang, China). BPI-9016M was dissolved in 0.5% carboxymethylcellulose sodium (CMC) for PDX treatment. For other experiments, the drug was dissolved in dimethyl sulfoxide at a stock concentration of 50 mM and stored at -80 °C. The DKK1 siRNA, miR103 mimic, miR203 mimic, miR103 inhibitor and miR203 inhibitor were constructed by RiboBio (Guangzhou, China) and the pENTER-DKK1 plasmid was purchased from Vigenebio (Maryland, USA). The transfections were performed according to the protocol provided by the manufacturer (Lipo3000, Thermo Fisher, MA, USA).

HIECs was transfected with miR-203 mimic, miR-203 AMOs and negative control respectively (RiboBio). The transfection reagents was added into Opti-MEM (31985070, Invitrogen) serum-free medium (100 nM) and transfected into HIECs using X-treme reagent (4476115001, Sigma). Cells were harvested 48 h after transfection to extract total RNA or protein. Transfection reagents and X-treme were separately mixed with 25 μL of Opti-MEM. Then blend the two mixtures and incubate for 20 min at room temperature. The mixture was added to the cells and incubated at 37°C for 5 h. Oligonucleotides for transfection are shown in Table S1.