Rabbit anti h3k9ac

Primary antibodies used in this study included: rabbit anti-(tetra)-acetyl histone H4 K5, 8, 12, 16ac (H4Kac4) (Millipore; note: according the manufacturer's material data sheet this antibody may cross-react with other acetylated histones like H2B); rabbit anti-H3K9ac (Diagenode); rabbit anti-PfHP1 (Brancucci et al., 2014 (link)); rabbit IgG antibody (Millipore); mouse/rabbit anti-Pfs230 (Ngwa et al., 2013 (link); Simon et al., 2016 (link)), rabbit anti-Pfs25 (BEI Resources); mouse anti-Pf39 (Scholz et al., 2008 (link)); rabbit anti-HA (Sigma Aldrich); mouse anti-proteasome SU α5 (Aminake et al., 2012 (link)), and anti-PfActinI (Ngwa et al., 2013 (link)). Mouse anti-PfRNF1 was generated for this study (see below). For indirect immunofluorescence assays (IFAs), the following dilutions of the antibodies were used: anti-H4KAc4 (1:200), anti-H3K9ac (1:200), mouse/rabbit anti-Pfs230 (1:200), anti-Pfs25 (1:1,000), anti-PfRNF1 (1:20), anti-PfHP1 (1:300), and anti-proteasome SU α5 (1:50). For Western blot (WB) analysis the following dilutions were used: anti-H4Kac4 (1:1,000), anti-H3K9ac (1:1,000), anti-Pf39 (1:1,000), anti-PfActinI (1:200), anti-PfRNF1 (1:200), rabbit anti-HA (1:1,000). For chromatin immunoprecipitation (ChIP) assays, 1 μg of each antibody (anti-H4Kac4, anti-H3K9ac, anti-PfHP1, IgG) was used.

Primary antibodies: rabbit homemade anti-TgHDAC3 described in Bougdour et al. (2009 (link)); RRID:AB_2713903), mouse anti-HA (Roche, RRID:AB_2314622), rabbit anti-H4K8ac (Millipore, RRID:AB_310524), rabbit anti-H4K12ac (Millipore, RRID:AB_11215637), rabbit anti-H3K4ac (Diagenode, RRID:AB_2713904), rabbit anti-H3K9ac (Diagenode RRID:AB_2713905), rabbit anti-H3K14ac (Diagenode, RRID:AB_2713906), rabbit anti-H3K18ac (Diagenode, RRID:AB_2713907), rabbit anti-H3K14ac (Millipore, RRID:AB_1977241) and mouse anti-H3K27ac (Diagenode, RRID:AB_2713908), anti-H4K20me3 (Diagenode, RRID:AB_2713909), anti-H3K9me3 (Millipore, RRID:AB_916348), anti-H3K4me1 (Diagenode, RRID:AB_2637078) and anti-H3K4me3 (Diagenode, RRID:AB_2616052). Western blot secondary antibodies were conjugated to alkaline phosphatase (Promega), whereas immunofluorescence secondary antibodies were coupled with Alexa Fluor 488 or Alexa Fluor 594 (Thermo Fisher Scientific). We also raised homemade H4K31acetylation- and H4K31monomethylation-specific antibodies in rabbit against linear peptides corresponding to amino acid residues 23–35 of histone H4 and carrying modified residue K31: C-DNIQGITKme1PAIR; C-DNIQGITKacPAIR and C-RDNIQGITKacPAIR. They were produced by Eurogentec and used for immunofluorescence, immunoblotting and chromatin immunoprecipitation.