C. difficile VPI 10463 was inoculated in anaerobic chopped meat media tubes (Hardy Diagnostics) injected with vancomycin (10 μg/mL), 2-DG (1 μg/mL, 10 μg/mL or 100 μg/mL), or untreated, and incubated for 8 h at 37 °C. After incubation, samples were serially diluted and plated in C. difficile agar base (Oxoid) plates supplemented with 7% laked horse blood (Lampire Biological Laboratories) and C. difficile selective supplement (Oxoid) and incubated anaerobically. Colonies were counted 3 days after culture.
C difficile selective supplement
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The C. difficile selective supplement is a laboratory product used to aid in the growth and identification of Clostridium difficile bacteria. It contains a combination of antimicrobial agents that inhibit the growth of other bacteria, allowing for the selective isolation of C. difficile.
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Lab products found in correlation
2 protocols using c difficile selective supplement
1
Vancomycin and 2-DG Effects on C. difficile
2
Co-culture Biofilm Generation
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For generation of co-culture biofilms, both C. difficile and B. fragilis were diluted to an OD600 of 1. Both species were diluted 1:100 into fresh BHIS with 0.1 M glucose. Biofilms assays were performed as described above and measured by a combination of CV staining and CFU. To distinguish between C. difficile and B. fragilis, serial dilutions used for determining CFU were plated on BHIS plates additionally supplemented with C. difficile selective supplement (Oxoid, UK). Colonies can be differentiated by size and colony morphology as B. fragilis form very small colonies.
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