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Cx 33

Manufactured by Olympus
Sourced in Japan

The Olympus CX-33 is a compound microscope designed for laboratory use. It features binocular viewing and a range of objectives for magnification. The CX-33 is built to provide clear, high-quality images for various scientific applications.

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4 protocols using cx 33

1

Cenomanian Amber from Myanmar: Microscopic Examination

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The amber specimen herein studied is from the Hukawng Valley in Tanai Township, Kachin State, northern Myanmar, and it is earliest Cenomanian in age, 98.8 ± 0.62 million years, by U-Pb dating of zircons from the volcanoclastic matrix of the amber [18 (link),19 (link)].
Observations were made by using an Olympus CX-33 (Olympus Imaging Corporation, Tokyo, Japan) light microscope. Photographs and drawings were taken by using a Sony Alpha 7II (Sony Corporation, Tokyo, Japan) digital camera attached to the Olympus CX-33. The figures were prepared with Adobe Photoshop 24.0.0 (Adobe, San Jose, CA, USA).
The morphological terminology follows Yoshizawa [20 ]. Abbreviations for body parts measured are: f1-fn: flagellomeres 1-n; mx2: second maxillary palp; mx4: fourth maxillary palp; ft1, ft2, ft3: first, second, and third tarsomere of foreleg; mt1, mt2, mt3: first, second, and third tarsomere of midleg; ht1, ht2, ht3: first, second, and third tarsomere of hindleg.
This manuscript has been registered in ZooBank under the number urn:lsid:zoobank.org:pub:683E3489-FE07-4E6D-9049-C28921BCAC53.
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2

Puparia of New Murraya exotica Species

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Puparia of the new species were collected on Murrayaexotica trees in the Maolan National Nature Reserve, Guizhou, China; no adults were collected in the samples. The puparia were mounted following the method suggested by Dubey and David (2012) . The terminology for morphological structures follows Bink-Moenen (1983) , Martin (1985) , and Gill (1990) . The habitus images were taken using a digital camera Nikon D500 and Keyence VHX-6000 digital microscope from
Guizhou University (GZU). Puparial measurements and microphotographs were taken using an Olympus (cx33) from
Zhejiang Agriculture and Forestry University, Lin’an, China (ZAFU).
The scanning electron microscope images were taken with a Hitachi SU8010 Scanning Electron Microscope (Hitachi, Japan) from Center of Electron Microscopy, ZAFU. Adobe Photoshop software was used to make small adjustments and to assemble the plates. The holotype is deposited in the Insect Collection of ZAFU.
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3

Immunofluorescent Analysis of Sciatic Nerve

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The paraffin-embedded sciatic nerve sections were incubated at 4 °C overnight with the following primary antibodies: anti-S100β (1:100, Abcam, USA), anti-CD-3 (1:150, Abcam, USA), anti-Ki67 (1:500, Abcam, USA), anti-C-caspase-3 (1:100, Abcam, USA), anti-MBP (1:1000, Abcam, USA), anti-β3-Tubulin (1:2000, Abcam, USA), anti-GAP43 (1:100, Abcam, USA), and DAPI (1:1000, Abcam, USA) was used to stain the nuclei. The results were measured by Fluorescence Microscope and Optical Microscope (Olympus CX33, Japan).
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4

H&E Staining for Visualizing Tissue Damage

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H&E staining was used to visualize tissue damage as previously described (33 (link)). Briefly, the brain tissues were dehydrated through an ethanol gradient from 70 to 100% and cleared with three changes of xylene (1 min for each change). The dehydrated tissues were embedded in paraffin, sectioned to a thickness of 20 µm, dewaxed in xylene overnight and subsequently passed through 3×10 min 99% ethanol and 2×10 min 96% ethanol and rehydrated. The sections were immersed in an aqueous hematoxylin solution (cat. no. H9627, MilliporeSigma) for 3 min at 25°C, differentiated with hydrochloric acid for 15 sec, rinsed briefly and counterstained with eosin (cat. no. 318906, MilliporeSigma) for 3 min at 25°C. After rinsing thoroughly in distilled water and dehydrated as described above, the slides were cleared with xylene, sealed and examined under a microscope (Olympus CX33, Olympus Corporation).
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