SK-N-SH cells grown in 6-well dishes were fixed with 4% paraformaldehyde for 30 min and then washed three times in PBS. Cells were incubated with the primary rabbit SARS-COV-2 Spike antibody (Abclonal, Wuhan, China) overnight at 4 °C and then incubated with Alexa Fluor 594 goat anti-rabbit antibody (Bioss, Woburn, MA, USA) for another 1 h. Cells were then counterstained with DAPI (US Everbright Inc., Suzhou, China) to visualize the nucleus morphology. Cells were mounted and photographed using BX41 fluorescence microscopy (Olympus, Tokyo, Japan) [18 (link)].
Sars cov 2 spike antibody
Manufactured by ABclonal
Sourced in China
The SARS-COV-2 Spike antibody is a laboratory reagent used for the detection and analysis of the SARS-CoV-2 virus spike protein. It is a specific antibody that can bind to the spike protein, allowing researchers to identify and study the presence of the virus in samples.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 594 goat anti rabbit antibody, by Bioss Antibodies (1 mentions)
Bx41 fluorescence microscopy, by Olympus (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by US Everbright (1 mentions)
Hrp labeled goat anti mouse igg h l, by Beyotime (1 mentions)
Ifnar1 antibody, by Abcam (1 mentions)
Mavs antibody, by Santa Cruz Biotechnology (1 mentions)
Irf3 antibody, by Proteintech (1 mentions)
Tbk1 antibody, by ABclonal (1 mentions)
β actin antibody, by Beyotime (1 mentions)
Hrp labeled goat anti rabbit igg h l, by Beyotime (1 mentions)
2 protocols using sars cov 2 spike antibody
1
Immunofluorescence Staining of SARS-CoV-2 Spike
2
Comprehensive Analysis of SARS-CoV-2 Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blotting analysis of cell lysates were performed as previously described (Zha et al., 2023 ). SARS-CoV-2 N Protein antibody (Cat# A20142; 1:1 000), SARS-CoV-2 3CLpro antibody (Cat# A20198; 1:1 000), SARS-CoV-2 Spike antibody (Cat# A20497; 1:1 500), Hsp90α/β antibody (Cat# A5027; 1:1 000), TBK1 antibody (Cat# A3458; 1:1 000), phospho-IRF3-S386 antibody (Cat# AP0995; 1:1 000) and ISG15 antibody (Cat# A2416; 1:1 000) were provided by ABclonal (Wuhan, China). IFNβ antibody (Cat# A5526; 1:1 000) was purchased from Selleck (Houston, TX, USA). MAVS antibody (Cat# sc-166583; 1:1 000) was provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). IRF3 antibody (Cat# 66670–1-Ig; 1:1 000) was obtained from Proteintech (Wuhan, China). IFNAR1 antibody (Cat# ab45172; 1:1 000) was purchased from Abcam (Cambridge, MA, USA). β-actin antibody (Cat# AF0003; 1:1 000), HRP-labeled goat anti-mouse IgG(H + L) (Cat# A0216; 1:5 000) and HRP-labeled goat anti-rabbit IgG(H + L) (Cat# A0208; 1:5 000) were obtained from Beyotime (Shanghai, China).
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