Melanoma cell lines A375 and SK-MEL-28 originated from primitive melanoma and MeWo originated from metastatic melanoma; cell lines were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). Cells were grown in a humidified atmosphere at 37 °C in 95% air, 5% CO2 in DMEM (Lonza BioWhitaker, Illkirch-Graffenstaden, France) for A375 and SK-MEL-28 cells and MEM (Lonza BioWhitaker) for MeWo cells supplemented with L-glutamine (2 mM) and 10% heat-inactivated Fetal Bovine Serum (FBS) for normoxic conditions. The induction of hypoxia was achieved by using 260 μM of the hypoxia mimetic desferrioxamine mesylate (DFX) (Sigma, Paris, France). After cells had grown to a confluence of 70%, the medium was changed and cells were incubated with new medium containing 260 μM DFX for 24 and 48 h.
Desferrioxamine mesylate dfx
Manufactured by Merck Group
Sourced in France
Desferrioxamine mesylate (DFX) is a laboratory-grade chemical compound. It functions as a chelating agent, capable of binding and sequestering metal ions, particularly iron. DFX is commonly used in various research and analytical applications involving the management and study of metal ion concentrations.
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Lab products found in correlation
2 protocols using desferrioxamine mesylate dfx
1
Hypoxia Induction in Melanoma Cell Lines
2
Hypoxia Induction in Mesothelioma Cell Lines
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The cell lines H2452 and H2052 (epithelial mesothelioma), MSTO-211H (biphasic mesothelioma), and MeT-5A (epithelial virus transformed, obtained from mesothelium of noncancerous individuals) were procured from the American Type Culture Collection (Rockville, MD). H2452, H2052, and MSTO-211H cells were cultured in RPMI-1640 (Roswell Park Memorial Institute-1640) medium supplemented with 10% heat-inactivated fetal bovine serum. MeT-5A cells were grown in defined medium (M199) as recommended by the American Type Culture Collection. Cells were cultured at 37 C in 20% O 2 and 5% CO 2 for normoxic conditions. Induction of hypoxia was achieved by using the hypoxia mimetic 260 mM desferrioxamine mesylate (DFX) (Sigma, Paris, France). MPM and MeT-5A cells were grown to 70% confluence. The medium was changed, and the cells were incubated with new medium containing 260 mM DFX for 24 and 48 hours.
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