Ek1296

Manufactured by Boster Bio

EK1296 is a lab equipment product. It is a device used for laboratory analysis and experimentation. The core function of EK1296 is to facilitate various laboratory procedures and processes.

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Lab products found in correlation

Proteome profiler mouse xl cytokine array, by R&D Systems (2 mentions) Dang10, by R&D Systems (2 mentions) Imagequant las 4000, by GE Healthcare (1 mentions) Imagequant las 4000 imaging system, by GE Healthcare (1 mentions) Imagequant tl, by GE Healthcare (1 mentions)

2 protocols using ek1296

Cytokine and ANGPT1 Profiling in Platelets

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Cytokine profiling assays were performed using the Proteome Profiler Mouse XL Cytokine Array (catalo no. ARY028; R&D Systems) according to the manufacturer’s protocol. Briefly, after isolating and activating the platelets of Pf4-Cre⁺Gucy1b1^+/LoxP and Pf4-Cre⁺Gucy1b1^LoxP/LoxP mice by shaking in RPMI as described above, samples were added to the supplied antibody-spotted nitrocellulose membrane and incubated at 4 °C overnight. Captured proteins were detected using a mixture of biotinylated detection antibodies followed by streptavidin-HRP and visualized using chemiluminescent detection reagents. Signal intensities were detected by an ImageQuant LAS 4000 imaging system and analyzed using the appropriate image analysis software (ImageQuant LAS TL, version 8.1; GE Healthcare Life Sciences). The signal intensities of target proteins were normalized to the signal intensities for the reference spots in each sample. The procedure was repeated for a total of six samples per group.
ANGPT1 ELISAs were performed to determine murine (catalog no. EK1296; Boster Biological Technology) and human (catalog no. DANG10; R&D Systems) protein levels according to the manufacturer’s recommendations.

Mauersberger C., Sager H.B., Wobst J., Dang T.A., Lambrecht L., Koplev S., Stroth M., Bettaga N., Schlossmann J., Wunder F., Friebe A., Björkegren J.L., Dietz L., Maas S.L., van der Vorst E.P., Sandner P., Soehnlein O., Schunkert H, & Kessler T. (2022). Loss of soluble guanylyl cyclase in platelets contributes to atherosclerotic plaque formation and vascular inflammation. Nature cardiovascular research, 1(12), 1174-1186.

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Proteome Profiling and Angiopoietin-1 ELISA

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Proteome profiling assays were performed using the Proteome Profiler Mouse XL Cytokine Array (ARY028, R&D Systems) according to the manufacturer's protocol. Briefly, after isolating and activating platelets of Pf4-Cre + Gucy1b1 +/flox mice and Pf4-Cre + Gucy1b1 flox/flox mice by shaking in RPMI as described above, samples were added to the supplied antibodyspotted nitrocellulose membrane and incubated at 4°C overnight. Captured proteins were detected using a mixture of biotinylated detection antibodies followed by streptavidinhorseradish peroxidase and visualized using chemiluminescent detection reagents. Signal intensities were detected by an ImageQuant LAS 4000 imaging system (GE Healthcare Life Sciences, Freiburg, Germany) and analyzed using the appropriate image analysis software (ImageQuant TL; GE Healthcare Life Sciences). Signal intensities of target proteins were normalized to signal intensities for reference spots in each sample. The procedure was repeated for a total of 6 samples per group.
Angiopoietin-1 enzyme-linked immunosorbent assays (ELISA) were performed to determine murine (EK1296, Boster Biological Technology, Pleasanton, CA) and human (DANG10, R&D systems) protein levels according to the manufacturer's recommendations.

Mauersberger C., Sager H.B., Wobst J., Dang T.A., Lambrecht L., Koplev S., Stroth M., Bettaga N., Schlossmann J., Wunder F., Friebe A., Björkegren J., Dietz L., Sandner P., Soehnlein O., Schunkert H., & Kessler T. (2021). Loss of soluble guanylyl cyclase in platelets contributes to atherosclerotic plaque formation and vascular inflammation.

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