Young adult Sprague-Dawley rats received from Charles River (Kisslegg, Germany) were housed on a 12 h light-dark cycle with ad libitum access to water and food.
Young adult sprague dawley rats
Manufactured by Charles River Laboratories
Sourced in Germany, United States
Young adult Sprague Dawley rats are laboratory animals commonly used in a variety of research applications. They are characterized by their docile temperament and reliable breeding performance. These rats are widely accepted as a standard model organism for studying various biological processes and disease conditions.
Automatically generated - may contain errors
3 protocols using young adult sprague dawley rats
1
Sprague-Dawley Rat Housing Conditions
2
Sprague Dawley Rat-Derived Cell Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Young adult Sprague Dawley rats (200–250 g, Charles River Laboratories, Wilmington, MA, USA) were used for bone marrow extraction. OPs were harvested from neonatal Sprague Dawley rats for comparison of marker expression with aMSC-derived OPs. Dorsal root ganglia were harvested from embryonic day 15 Sprague Dawley rats. Homozygous shiverer mice (C3Fe.SWV-MBPShi/J, The Jackson Laboratory, Bar Harbor, ME, USA) were used as recipient of aMSC-derived OPs to demonstrate in vivo myelination as a result of OP transplantation into the corpus callosum. C57BI/6J mice (The Jackson Laboratory) were used to illustrate degree of myelination in the wild type. All experimental protocols were approved by the Committee for Use of Live Animals in Teaching and Research, The University of Hong Kong.
3
Hippocampal Slice Incubation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Young adult Sprague Dawley rats (age 4 -10 weeks; Charles River Labs, Wilmington MA) were anaesthetized with isoflurane, decapitated, and brains removed. 300μm coronal slices from hippocampus were made on a VT1000P vibratome (Leica Biosystems, Buffalo Grove, IL) in oxygenated (95%O2/5%C02) ice-cold high sucrose cutting solution (in mM: 85.0 NaCl, 2.5 KCl, 4.0 MgSO4, 0.5 CaCl2, 1.25 NaH2PO4, 25.0 Glucose, 75.0 Sucrose) and stored in a submersion chamber with continuously oxygenated standard ACSF (in mM: 119.0 NaCl, 2.5 KCl, 1.3 MgSO4, 2.5 CaCl2, 1.0 NaH2PO4, 26.0 NaHCO3, 11.0 Glucose). Slices were incubated in an interface chamber with either AM:MMA Polymer 2 or vehicle (DMSO) for 1 or 3 hours.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!