Human alt elisa kit

AlkP and ALT levels in murine sera were analysed using the Human ALKP ELISA Kit (Thermo Fisher Scientific, #EH19RB) and Human ALT ELISA Kit (#ab234578, Abcam, UK), respectively, following the manufacturer’s specifications.
Results were expressed as U/L.

LDH levels in vascular and hepatic medium supernatants were measured by using the Cytotoxicity Detection Kit^PLUS (LDH) (Roche, Basel, Switzerland). Therefore, samples were collected from drug-treated liver models every 24 h for up to 72 h. Supernatants were directly diluted 1:2 in LDH storage buffer containing 200 mM Tris (Carl Roth)-HCL (VWR International, Darmstadt, Germany) at pH 7.5, 10% glycerol (Sigma-Aldrich), 1% bovine serum albumin (Sigma-Aldrich) and AQUA AD iniectabilia to conserve LDH activity during freezing. Samples were stored at − 20 °C until performing the assay. Samples were thawed and the assay was performed according to the manufacturer’s instruction. LDH activity was analyzed spectrophotometrically at 490 nm and concentrations were calculated from a standard curve obtained from dilutions of the LDH standard (Tecan Reader infinite F201, Tecan).
The release of ALT into hepatic and vascular culture supernatants was analyzed using the Human ALT ELISA Kit (Abcam). The assay was performed according to the manufacturer’s protocol in a 384-well format with sample and reagent volumes accordingly adopted. ALT levels were determined spectrophotometrically at 450 nm and concentrations were calculated from an ALT standard curve (Tecan Reader infinite F201, Tecan).