Ints11

Manufactured by Merck Group

INTS11 is a laboratory instrument designed for the detection and quantification of specific biomolecules. It utilizes advanced optical technology to perform sensitive and reliable analyses. The core function of INTS11 is to provide accurate and reproducible measurements for research and diagnostic applications.

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Lab products found in correlation

2 protocols using ints11

Comprehensive Protein Expression Analysis

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Western blotting experiments and/or IF and IF combined to FISH were performed using the following primary antibodies: PSPC1 (Sigma-Aldrich, SAB4200503), PSF (Sigma-Aldrich, P2860), FBXO11 (Novus Biologicals, NB100-59826), TCF7L2 (Cell Signaling Technology, 2565), CPSF1 (Santa Cruz Biotechnology, sc-166281), CPSF2 (Santa Cruz Biotechnology, sc-165983), GAPDH (Abcam, ab9485), NONO (Bethyl Laboratories, A300-587A), TDP-43 (ProteinTech, 12892-1 ap), CPSF3L or INTS11 (Sigma-Aldrich, A107128), INTS1 (Millipore), INIP [C9orf80 (E-12), Santa Cruz Biotechnology, SC-137357], INTS3 (Bethyl Laboratories, A300-427A), INTS6 (Bethyl Laboratories, A301-658A), INTS8 (Bethyl Laboratories, A300-269A), p53-DOI (Santa Cruz Biotechnology, sc-126), p21 (Santa Cruz Biotechnology, sc-6246), phospho-γH2AX (Cell Signaling Technology, 2577), Laminin A + C (Abcam, ab108922), vinculin (Sigma-Aldrich, V9131), GFP (Clontech, 632375), and H3 (Abcam, ab1791).

Barra J., Gaidosh G.S., Blumenthal E., Beckedorff F., Tayari M.M., Kirstein N., Karakach T.K., Jensen T.H., Impens F., Gevaert K., Leucci E., Shiekhattar R, & Marine J.C. (2020). Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1. Science Advances, 6(27), eaaz9072.

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Antibody-based CHIP and Immunoblot Assays

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Antibodies used for CHIP and immunoblot included INTS11 (Bethyl Laboratories, A301-274A), INTS11 (Sigma, HPA029025), RNAPII (Santa Cruz Biotechnology, sc-899), GAPDH (Santa Cruz Biotechnology, sc-25778), phospo-ERK1/2 (Cell Signaling Technology, nos. 4370 and 9101; and Invitrogen, catalog no. 700012), ERK1/2 (Cell Signaling Technology, no. 9102), MED1 (Bethyl Laboratories, A300-793A), MED12 (Bethyl Laboratories, A300-774A), phospho-p90RSK (Thr359) (Cell Signaling Technology, no. 8753), RSK1 (Cell Signaling Technology, no. 9333), phosphor-EGFR (pTyr1068) (Cell Signaling Technology, no. 3777), EGF receptor (Cell Signaling Technology, no. 4267), and phosphothreonine (Cell Signaling Technology, no. 9381). Flag M2-conjugated beads (Sigma, A2220) were used for immunoprecipitation.

Yue J., Lai F., Beckedorff F., Zhang A., Pastori C, & Shiekhattar R. (2017). Integrator orchestrates RAS/ERK1/2 signaling transcriptional programs. Genes & Development, 31(17), 1809-1820.

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