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Ebiosn3

Manufactured by Thermo Fisher Scientific
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The EBioSN3 is a high-performance laboratory instrument designed for DNA sequencing applications. It provides reliable and accurate sequencing data by utilizing advanced optical detection and data processing technologies. The core function of the EBioSN3 is to enable efficient and accurate DNA sequencing for research and diagnostic purposes.

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Lab products found in correlation

Vu 1d9, by Abcam (2 mentions) Egfr1, by Abcam (2 mentions) Lsrfortessa, by BD (1 mentions) Aria 2, by BD (1 mentions)

3 protocols using ebiosn3

1

Immunohistochemical Analysis of CRC Markers

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Tumoural and non-neoplastic tissue sections from CRC patients were subjected to immunohistochemical staining (Fig. 3a and Supplementary Fig. 24). Primary monoclonal antibodies against EGFR (EGFR1, Abcam, 1:50 dilution), EpCAM (VU-1D9, Abcam, 1:100 dilution), and CD24 (eBioSN3, ebioscience, 1:100 dilution) were used and the staining was conducted on a Ventana BenchMark XT automated slide stainer (Ventana Medical Systems, Inc.), according to the manufacturer’s recommendations. Stained images were scored, based on the fraction of positive cells among total cancer cells, by a pathologist (G.Y.) who was blind to clinicopathological variables and EV profiling results. Positive cells were defined by positively stained cytoplasmic or membranous pattern within cancer cells in the face of concurrent negative labelling in non-neoplastic tissues. The marker expression was ranked from 0 (negative) to 1 (positive) with the increment of 0.1; this level was used as an ordinal variable in Spearman’s rank test with EV profiling results.
Park J., Park J.S., Huang C.H., Jo A., Cook K., Wang R., Lin H.Y., Deun J.V., Li H., Min J., Wang L., Yoon G., Carter B.S., Balaj L., Choi G.S., Castro C.M., Weissleder R, & Lee H. (2021). A high-throughput magneto-electrochemical array for the integrated isolation and profiling of extracellular vesicles from plasma. Nature biomedical engineering, 5(7), 678-689.
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2

Immunohistochemical Analysis of CRC Markers

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Tumoural and non-neoplastic tissue sections from CRC patients were subjected to immunohistochemical staining (Fig. 3a and Supplementary Fig. 24). Primary monoclonal antibodies against EGFR (EGFR1, Abcam, 1:50 dilution), EpCAM (VU-1D9, Abcam, 1:100 dilution), and CD24 (eBioSN3, ebioscience, 1:100 dilution) were used and the staining was conducted on a Ventana BenchMark XT automated slide stainer (Ventana Medical Systems, Inc.), according to the manufacturer’s recommendations. Stained images were scored, based on the fraction of positive cells among total cancer cells, by a pathologist (G.Y.) who was blind to clinicopathological variables and EV profiling results. Positive cells were defined by positively stained cytoplasmic or membranous pattern within cancer cells in the face of concurrent negative labelling in non-neoplastic tissues. The marker expression was ranked from 0 (negative) to 1 (positive) with the increment of 0.1; this level was used as an ordinal variable in Spearman’s rank test with EV profiling results.
Park J., Park J.S., Huang C.H., Jo A., Cook K., Wang R., Lin H.Y., Deun J.V., Li H., Min J., Wang L., Yoon G., Carter B.S., Balaj L., Choi G.S., Castro C.M., Weissleder R, & Lee H. (2021). A high-throughput magneto-electrochemical array for the integrated isolation and profiling of extracellular vesicles from plasma. Nature biomedical engineering, 5(7), 678-689.
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3

Flow Cytometry Analysis of HMLER Cells

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Briefly, all cells were harvested for flow cytometry using the following protocol. Cells were trypsinized and neutralized with growth medium containing 10 % FBS and the medium was removed. Cells were resuspended in HBSS+ (HBSS + 2 % FBS + HEPES buffer) and passed through a 40-μm mesh filter.
For antibody staining of HMLER cells, anti-human CD24-APC (eBioscience, clone eBioSN3, ref. 17-0247-42) and anti-human/mouse CD44-eFluor 450 (eBioscience, clone IM7, 48-0441-82) were diluted in HBSS+ at 1:50 dilution and used to resuspend cells. Cells were incubated for 30 min at 4 °C, rinsed twice in HBSS+, resuspended in HBSS+, and filtered through a 40-μm mesh filter.
Flow cytometry analysis was performed on BD LSR Fortessa. Sorting was performed on BD AriaII. FlowJo was used for the analysis.
Toneff M.J., Sreekumar A., Tinnirello A., Hollander P.D., Habib S., Li S., Ellis M.J., Xin L., Mani S.A, & Rosen J.M. (2016). The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states. BMC Biology, 14, 47.
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