Anti glial fibrillary acid protein

Anti-hypoxia-inducible factor (HIF)-1α and anti-aldehyde dehydrogenase (ALDH) 1 antibodies were purchased from BD Biosciences (San Jose, CA, USA). Anti-CD34, anti-CD44s, anti-glial fibrillary acid protein (GFAP), and anti-human smooth muscle actin (SMA) antibodies were obtained from Dako (Copenhagen, Denmark). Anti-Snail and anti-Slug antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-Sox2, anti-S100A4 (rabbit), anti-Twist 1, anti-Nestin, and anti-CD44v6 antibodies were obtained from Abcam (Cambridge, MA, USA). Anti-S100A4 (mouse), anti-carbonic anhydrase (CA) 9, and anti-NMIIA antibodies were from Proteintech (Rosemont, IL, USA). Anti-ZEB 1 and anti-β-actin antibodies were from Sigma-Aldrich Chemicals (St Louis, MA, USA). Blebbistatin and cobalt chloride (CoCl₂) were purchased from Toronto Research Chemicals (North York, ON, Canada) and Sigma Chemical, respectively.

94-3A10, 71E10, 9C10, and 33A-3F3 monoclonal anti-αS specific mouse antibodies were previously generated and described in Dhillon et al. 2017 [12 (link)]. 9C10 preferentially reacts with aggregated αS. αS phospho-Ser129 (pSer129) antibody 81A was previously characterized [31 (link), 50 (link)] and EP1536Y was obtained from Abcam (Cambridge, MA). Rabbit antibody to p62 (SQSTM1; Proteintech, Chicago, IL) is a general marker of inclusion pathology. Anti-glial fibrillary acid protein (GFAP) (Dako, Agilent, Carpentaria, CA), and CD11B (Abcam, Cambridge, MA) are reactive for astrocytes or microglia, respectively. Rabbit antibody to tubulin polymerization-promoting protein/p25α (Novus Biologicals, Centennial, CO) was obtained from Fisher Scientific.