The expression levels of Foxp3 and CD25 receptor protein were examined immunohistochemically in pericardial fluid biopsy samples obtained from the patients with CHF and NHF. Staining of CD25 (1:100; ab61195, Abcam, Cambridge, MA, USA) and Foxp3 (1:100; sc-28705, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) immunohistochemistry was performed on formalin-fixed sections, followed by incubation with PV-6001 (Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China) according to the manufacturer's instructions. Each stained histological section was examined under an Olympus BX53F microscope (Olympus Corporation) connected to a computerized image-analysis system (Image-Pro v6.0; Media Cybernetics Inc., Silver Spring, MD, USA).
Sc 28705
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom
Sc-28705 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for use in scientific research and analysis applications. The core function of this product is to provide a specific tool or apparatus for researchers to utilize in their work, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
3 3 diaminobenzidine (dab), by Agilent Technologies (2 mentions)
H 190, by Santa Cruz Biotechnology (2 mentions)
Ab6671, by Santa Cruz Biotechnology (2 mentions)
Ab79056, by Abcam (2 mentions)
Ab7737, by Abcam (2 mentions)
Bx53f microscope, by Olympus (1 mentions)
Image pro v 6, by Media Cybernetics (1 mentions)
Ab61195, by Abcam (1 mentions)
Pv 6001, by Zhongshan Golden Bridge Biotechnology (1 mentions)
3 protocols using sc 28705
1
Immunohistochemical Analysis of Foxp3 and CD25 in CHF
2
Histopathological Analysis of Organ Damage in Mice Post-BMT
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Mice were sacrificed on day 28 after BMT and organs captured, cryoembedded, and sectioned. Tissue specimens were fixed in 10% formalin buffer and embedded in paraffin. Sections (6 μm thick) were stained with H&E and the histologic score was determined using established scoring systems [31 (link), 32 (link)]. For immunohistochemistry staining, sections were stained with primary antibodies against IL-6 [Abcam, Cambridge, England (ab7737)], IL-17 [Abcam (ab79056)], TNF-α [Abcam (ab6671)] and Foxp3 [H-190, Santa Cruz Biotechnology (sc-28705)] overnight at 4 °C, followed by addition of a biotinylated secondary antibody and a streptavidin-peroxidase mixture for 1 h. Color was developed by addition of 3,3-diaminobenzidine (Dako, Carpinteria, CA).
3
Histological and Immunohistochemical Evaluation of Organ Tissues
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Mice were sacri ced on day 28 after BMT and organs captured, cryoembedded, and sectioned. Tissue specimens were xed in 10% formalin buffer and embedded in para n. Sections (6 mm thick) were stained with H&E and the histologic score was determined using established scoring systems [31, 32] . For immunohistochemistry staining, sections were stained with primary antibodies against IL-6 (Abcam, Cambridge, England (ab7737)), IL-17 (Abcam (ab79056)), TNF-α (Abcam (ab6671)) and Foxp3 (H-190, Santa Cruz Biotechnology (sc-28705)) overnight at 4 °C, followed by addition of a biotinylated secondary antibody and a streptavidin-peroxidase mixture for 1 h. Color was developed by addition of 3,3diaminobenzidine (Dako, Carpinteria, CA).
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