Tacs annexin 5 fitc kit

His-tagged TRAIL for in vivo work was produced and purified as previously described [20 (link)]. The following chemicals or kits were used for assaying cell proliferation and apoptosis: MTT (AMRESCO, Solon, OH, USA) and TACS® Annexin V-FITC Kit (Gaithersburg, MD, USA). Reagents for SEM and TEM were obtained from Electron Microscopy Sciences (Hatfield, PA, USA): glutaraldehyde, osmium tetroxide and uranyl acetate. APC-conjugated antibodies specific for the extracellular domains of human CD41, CD42b, CD47, CD61 and CD62P in flow cytometry and fluorescence microscopy studies were purchased from Biolegend (San Diego, CA, USA). Primary CD41 antibodies for the extracellular domain (M-148) and cytoplasmic domain (B-9) detection and human CD47 blocking antibody (B6H12) were from Santa Cruz Biotech (Dallas, TX, USA).

The following chemicals or kits were used for assaying cell proliferation and apoptosis: MTT (AMRESCO, Solon, OH, USA) and TACS® Annexin V-FITC Kit (Gaithersburg, MD, USA). Reagents for TEM were obtained from Electron Microscopy Sciences (Hatfield, PA, USA): glutaraldehyde, osmium tetroxide and uranyl acetate. APC-conjugated antibodies for human and mouse CD41 and PE-conjugated antibodies for human TRAIL were purchased from Biolegend (San Diego, CA, USA). Primary antibodies for human TRAIL and β-actin were obtained from PeproTech (Rocky Hill, NJ, USA) and Santa Cruz Biotech (Santa Cruz, CA, USA). HRP-conjugated anti-mouse and anti-rabbit antibodies were from Santa Cruz Biotech.