Morphological characterization included the occurrence of specialized cells, such as heterocysts or akinetes, as well as the presence of sheaths, trichome characteristics or cell and colony morphology. This characterization was carried out using an Olympus BH2-RFCA photomicroscope (Olympus, Tokyo, Japan). The cyanobacterial characteristics of the natural samples as well as the cultures were compared with the information provided in the taxonomic keys of Komárek (2013) and Komárek and Anagnostidis (1999, 2005) [37 ,38 ,39 ].
Bh2 rfca photomicroscope
Manufactured by Olympus
Sourced in Japan
The BH2-RFCA photomicroscope is a high-performance optical microscope designed for advanced microscopy applications. It features a binocular observation tube, coaxial coarse and fine focusing controls, and a built-in reflected light illumination system. The BH2-RFCA is capable of providing clear, high-resolution images of a wide range of specimens.
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Lab products found in correlation
2 protocols using bh2 rfca photomicroscope
1
Cyanobacterial Morphological Characterization
2
Morphological Analysis of Rivularia-like Colonies
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Samples were inspected under a Leica MZ12.5 dissecting stereomicroscope (Leica, Leica Microsystems, Wetzler, Germany) equipped with epifluorescence and video camera systems and by an Olympus BH2-RFCA photomicroscope (Olympus, Tokyo, Japan) equipped with phase-contrast, epifluorescence, and video camera systems (Leica DC Camera, Leica Microsystems). To better observe the samples via microscopy, several colonies were decalcified with 0.5 M EDTA. Representative colonies of Rivularia-like samples were analyzed, except those with ambiguous morphologies, for which the variability of the morphotypes found within the colony precluded analysis. Size measurements were analyzed using SigmaScan Pro 5.0.0 software. Kruskal–Wallis one-way analysis of variance of ranks was used to determine the significance of differences between size measurements.
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